基于色差原理分析木香有效成分含量与颜色值的相关性

目的:通过测定木香粉末色差值及有效成分木香烃内酯、去氢木香内酯和挥发油的含量,将代表颜色的指标值与代表质量的指标值相关联,探讨有效成分含量与颜色值之间的相关性,为木香的质量评价提供依据。方法:利用色差计对木香粉末色差值进行测量,以流动相甲醇-水(65∶35)等度洗脱,检测波长225 nm,采用HPLC测定木香烃内酯、去氢木香内酯的含量,运用水蒸气蒸馏法测定木香挥发油的含量,通过SPSS 21.0软件进行相关性分析。结果:木香烃内酯、去氢木香内酯、挥发油含量与L*(代表颜色深浅),b*(代表颜色红绿方向),E*ab(代表总色差)存在负相关关系,且效果极显著(P0.01),但与a*(代表颜色红绿方向)不显著相关。结论:L*,b*,E*ab越小,3种有效成分的含量越高。木香颜色值与3种有效成分含量都有关,且颜色深的木香有效成分含量较高,质量较好。通过利用色差计对木香粉末的色差值进行测定,可以快速预测木香中3种有效成分的含量,可为该药材质量评价体系的建立提供参考。

Correlation Between Effective Components Content and Color Values of Aucklandiae Radix Based on Color Difference Principle

Objective: By measuring the color values of Aucklandiae Radix powder and the contents of costunolide,dehydrocostus lactone and volatile oil,the index value of the representative color was correlated with the index value representing the quality,to explore the correlation between contents of active ingredients and the color values, and provide basis for the quality evaluation of Aucklandiae Radix. Method: The color difference values of Aucklandiae Radix powder was measured by colorimeter.The contents of costunolide and dehydrocostus lactone were determined by HPLC,mobile phase was methanol-water(65:35),the detection wavelength was set at 225 nm.The content of volatile oil was determined by steam distillation method.The correlation analysis was carried out by SPSS 21.0 software. Result: There was a negative correlation between L^*(representing color shade),b^*(representing color red-green direction),E^*ab(representing total color difference) and contents of costunolide,dehydrocostus lactone,volatile oil(P<0.01). Conclusion: The color values of Aucklandiae Radix powder has a certain correlation with the content of costunolide,dehydrocostus lactone and volatile oil.The smaller the content of L^*,b^* and E^*ab,the higher the content of three active ingredients.The color is darker,the content of active ingredients in Aucklandiae Radix is higher and its quality is better.The contents of 3 active ingredients in Aucklandiae Radix can be quickly predicted by determining the color difference values,which can provide a new idea for quality evaluation of this herb.