23株非结核分枝杆菌药物敏感性试验分析

目的 对自贡市临床分离鉴定的23株非结核分枝杆菌分别进行26种抗生素的药物敏感试验,了解非结核分枝杆菌对不同抗生素的耐药性,为非结核分枝杆菌病临床治疗提供依据。方法 采用微孔板阿尔玛蓝测定法(microplate Alamarblue assay,MABA)测试每种药物对每株非结核分枝杆菌的最低抑菌浓度(MIC值),通过MIC值判断该菌对此种抗生素是否耐药。结果 非结核分枝杆菌对大部分抗结核药物耐药,并且对部分药物的耐药存在种间差异。其中脓肿分枝杆菌耐药率达84.6%,鸟分枝杆菌与胞内分枝杆菌分别达53.8%与52.3%,堪萨斯分枝杆菌耐药率最低为38%。结论 对临床非结核分枝杆菌肺病,快速菌株鉴定及药敏试验是治疗的关键。

Drug resistance of the 23 non-tuberculosis mycobacterium

Twenty-six kinds of antibiotics were used to test drug susceptibility for 23 strains of non-tuberculosis mycobacterium from Zigong City, China to investigate the drug resistance of non-tuberculosis mycobacterium to different antibiotics. We used the microplate Alamarblue assay to get the MIC of evert antibiotics, and we got through the MIC to judge whether the bacteria to antibiotic resistance. Most of the strains had drug resistance to various antituberculosis drug, and there had interspecific differences in drug resistance for some antibiotics. The resistance rate of Mycobacterium abscess was 84.6%, and for Mycobacterium avium and Mycobacterium intracellulare were 53.8% and 52.3%, respectively, and for the Mycobacterium kansasii was 38%. For non-tuberculosis mycobacterium in clinical disease, the key to treatment was fast strain identification and drug sensitive test.