亳白芍药材质量与土壤中主要无机元素相关性

目的:分析亳白芍药材质量和无机元素含量与其生长地土壤中无机元素的相关性。方法:采用电感耦合等离子体质谱法对亳州12个不同乡镇白芍药材及土壤中锌(Zn),锰(Mn),钾(K),铁(Fe),钙(Ca),镁(Mg),铜(Cu),镉(Cd)等19种无机元素含量进行测定,并用高效液相色谱发测定亳白芍中没食子酸、芍药苷、芍药内酯苷等5种主成分含量,同时测定其水浸出物量,运用SPSS 19.0软件分析其相互间的相关性。结果:12个乡镇,白芍药材中富含Fe,Mn,Zn,K,Ca,Mg共6种无机元素,其中K,Ca,Na的含量较高,Fe,Mn元素的含量次之,Zn元素含量较低;土壤中Cr,Hg,Cd,Cu含量与药材中含量呈显著性相关,Ca,Al,Ti,Mn等元素含量与药材的部分元素呈显著相关性,其他元素相关性不强;土壤中Na元素含量对白芍药材质量影响最大,与药材中没食子酸、芍药内酯苷、芍药苷、苯甲酸、苯甲酰芍药苷的含量及水浸出物量均呈负相关;重金属元素含量较低,都在2015年版《中国药典》规定范围之内。结论:通过分析药材质量和其中无机元素含量与土壤无机元素含量的相关性,为以后亳白芍生产区域的选择提供基础数据,为亳白芍GAP基地的建设提供参考。

Correlation Between Quality of Paeoniae Radix Alba in Bozhou and Main Inorganic Elements in Soil

Objective: To investigate the correlation between the quality and the contents of inorganic elements in Paeoniae Radix Alba in Bozhou. Method: The contents of nineteen elements,such as Zn,Mn,K,Fe,Ca,Mg,Cu,Cd in Paeoniae Radix Alba were collected from twelve towns in Bozhou, the corresponding soil were determined using inductively coupled plasma mass spectrometry (ICP-MS) and the contents of gallic acid,paeoniflorin and paeonolide were determined by using high performance liquid chromatography(HPLC). In addition,the contents of water-soluble extractives were also determined, and the correlation between them was analyzed by using SPSS 19.0 software. Result: In the twelve towns,Paeoniae Radix Alba had high contents of six kinds of inorganic elements,namely Fe,Mn,Zn,K,Ca and Mg. Among them,the contents of K,Ca and Na were the highest,the contents of Fe and Mn were the second,and the content of Zn was the lowest. The contents of Cr,Hg,Cd and Cu in soil were significantly correlated with the contents of inorganic elements, and the contents of other elements,such as Ca,Al,Ti and Mn, were significantly correlated with other elements in Paeoniae Radix Alba in Bozhou,while other inorganic elements in soil were not correlated. The content of Na in soil had the greatest impact on the quality of Paeoniae Radix Alba in Bozhou,and it was negatively correlated with the contents of gallic acid,paeoniflorin,paeonolide,benzoic acid,benzoyl paeoniflorin and water-soluble extractives. The contents of heavy metal elements were low,which was within the range on the basis of the 2015 Chinese Pharmacopoeia. Conclusion: By analyzing the correlation between the quality and the contents of inorganic elements in Paeoniae Radix Alba in Bozhou and the contents of the inorganic elements in corresponding soil,we can provide basic data and reference for the future choice of production areas and the construction of GAP bases of Paeoniae Radix Alba in Bozhou.