环介导等温扩增技术检测方法的研究进展

环介导等温扩增技术是一种可用于临床诊断的快速检测方法,可用于检测病毒、细菌、寄生虫等多种病原体。经不断完善,现已在临床检测过程中发挥重要作用。可用显色试剂对反应结果实现可视化,即结果的即时显示,无需后续验证。但是,无论是荧光染料还是显色指示剂,对反应过程都会产生或多或少的影响,目前研究表明羟基萘酚蓝是最好的显色试剂。同时,人们发现电化学技术也可以应用于此,使检测反应更快,操作更加简单。生物传感器可以对多种反应产物进行同步检测,而微流控芯片技术更是方便携带,有望成为实时检测技术的理想载体。

Advanced of the detection method of loop-mediated isothermal amplification

loop-mediated isothermal amplification is a rapid detection method that can be used for clinical diagnosis and can be used to detect viruses, bacteria, parasites and other pathogens. It has been improved and plays an important role in the clinical testing process. The color reaction can be used to achieve visualization of the results, and the results can immediately display. However, both the fluorescent dye and the color indicator can impact the reaction process. The current study shows that hydroxyl naphthol blue is the best color reagent. At the same time, it was found that electrochemical technology can also be applied to the method, which can make the detection reaction be faster and the operation more simple. Biosensors can synchronized detect a variety of reaction products. And microfluidic chip is easy to carry and is expected to become the ideal carrier for real-time detection technology.