| 基于UPLC-ESI-Q-TOF-MS的红禾麻提取物化学成分分析 |
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| 引用本文： | 唐娟,吴耽,陈思颖,李莹,李靖,巩仔鹏,李维维,兰燕宇,王永林.基于UPLC-ESI-Q-TOF-MS的红禾麻提取物化学成分分析[J].中国实验方剂学杂志,2018,24(24):67-72. |
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| 作者姓名： | 唐娟 吴耽 陈思颖 李莹 李靖 巩仔鹏 李维维 兰燕宇 王永林 |
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| 作者单位： | 贵州医科大学贵州省药物制剂重点实验室民族药与中药开发应用教育部工程研究中心药学院 |
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| 基金项目： | 国家自然科学基金项目（81560693）；贵州省科技厅科技创新人才团队建设项目（黔科合平台人才[2016]5613）；贵州省高层次创新型人才培养计划项目（黔科合平台人才[2016]5677；贵阳市科研创新团队项目（筑科合同[2017]30-29号） |
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| 摘 要： | 目的:建立超高效液相色谱-电喷雾-四级杆-飞行时间串联质谱联用技术(UPLC-ESI-Q-TOF-MS)研究红禾麻提取物中的主要化学成分。方法:采用Agilent Eclipse Plus C_(18)RRHD色谱柱(2.1 mm×100 mm,1.8μm);以0.1%甲酸水溶液-0.1%甲酸乙腈溶液为流动相进行梯度洗脱;流速0.25 m L·min~(-1),柱温40℃,进样量1μL。采用电喷雾离子源(ESI),负离子模式进行检测,运用Data Analysis软件,并结合保留时间、精确相对分子质量和相关文献进行数据分析。结果:通过结合对照品及文献报道的质谱数据对红禾麻提取物中化学成分进行分析,共分析鉴定13个化学成分,包括4个儿茶素类,3个苯丙素类和6个黄酮类,其中新绿原酸、隐绿原酸、绿原酸、芦丁、异槲皮苷、木犀草苷、山柰酚-3-O-芸香糖苷、紫云英苷和槲皮苷均首次在该植物中发现。结论:研究中建立的检测方法简便、准确,为快速鉴定分析化学成分提供一种有效的分析方法,并比较全面地阐明了红禾麻提取物中的化学成分,为其质量控制和药效物质基础研究奠定一定的基础。
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| 关 键 词： | 红禾麻 超高效液相色谱-电喷雾-四级杆-飞行时间串联质谱联用技术 儿茶素类 苯丙素类 黄酮类 |
| 收稿时间： | 2018/6/12 0:00:00 |
Identification of Chemical Compositions in Laportea bulbifera by UPLC-ESI-Q-TOF-MS |
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| TANG Juan,WU Dan,CHEN Si-ying,LI Ying,LI Jing,GONG Zi-peng,LI Wei-wei,LAN Yan-yu and WANG Yong-lin.Identification of Chemical Compositions in Laportea bulbifera by UPLC-ESI-Q-TOF-MS[J].China Journal of Experimental Traditional Medical Formulae,2018,24(24):67-72. |
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| Authors: | TANG Juan WU Dan CHEN Si-ying LI Ying LI Jing GONG Zi-peng LI Wei-wei LAN Yan-yu and WANG Yong-lin |
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| Institution: | Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutial Science, Guizhou Medical University, Guiyang 550004, China,Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutial Science, Guizhou Medical University, Guiyang 550004, China,Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutial Science, Guizhou Medical University, Guiyang 550004, China,Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutial Science, Guizhou Medical University, Guiyang 550004, China,Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutial Science, Guizhou Medical University, Guiyang 550004, China,Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutial Science, Guizhou Medical University, Guiyang 550004, China,Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutial Science, Guizhou Medical University, Guiyang 550004, China,Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutial Science, Guizhou Medical University, Guiyang 550004, China and Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutial Science, Guizhou Medical University, Guiyang 550004, China |
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| Abstract: | |
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| Keywords: | Laportea bulbifera UPLC-ESI-Q-TOF-MS catechin phenylpropanoids flavonoid |
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