黄红钻乙酸乙酯萃取部位化学成分分离鉴定

目的:研究"钻类"瑶族药"十八钻"之一的黄红钻乙酸乙酯萃取部位的化学成分。方法:黄红钻干燥药材经粉碎,用70%乙醇水进行回流提取,得到粗提物,粗提物加适量的水分散,分别用石油醚、乙酸乙酯、正丁醇进行萃取,对其中乙酸乙酯萃取部位采用反复硅胶柱色谱法、高效制备液相进行分离纯化,通过化合物的谱学数据分析(包括1H-NMR,13C-NMR,MS),以及比对文献数据进行结构鉴定及确证。结果:从黄红钻乙酸乙酯萃取部位分离出11个化合物,分别鉴定为对羟基苯甲醛(1),香草醛(2),松柏醛(3),4-羟基苯乙基乙酸酯(4),反式肉桂酸(5),反式阿魏酸(6),丁香酸乙酯(7),4-羟基-3,5二甲氧基苯甲醛(8),反式阿魏酸乙酯(9),4-羟基-3-甲氧基苯甲酸乙酯(10),邻苯二甲酸-二(2-乙基-己基)酯(11)。结论:从黄红钻中分离得到的化合物大部分为小分子的酚酸类化合物,且所有的化合物均为从黄红钻中首次分离得到。以上结果为黄红钻药效物质基础的研究提供了依据。

Chemical Constituents from Ethyl Acetate Extract of Jasminum pentaneurum

Objective: To study the chemical constituents of ethyl acetate extract from one of the "Shi ba zuan" of Yao medicines, Jasminum pentaneurum. Method: The crude extract of dry powder from stems of J. pentaneurum was obtained by using 70% ethanol water refluxing;the extract was evaporated under vacuum to obtain the dry residue. Then, the residue was dissolved in water and extracted with petroleum ether, ethyl acetate, and n-butanol, respectively. The compounds from the ethyl acetate fraction were isolated and purified by repeated silica gel column and high performance liquid chromatography (HPLC), and their structures were determined by spectroscopic data analyses, including ¹H-NMR, ¹³ C-NMR, and MS, as well as by comparison with the data from literature. Result: Eleven compounds were obtained and identified as 4-hydroxybenzyl aldehyde(1), vanillin(2), coniferaldehyde(3), 4-hydroxy phenethyl acetate(4), trans-cinnamic acid(5), trans-ferulic acid(6), syringic acid ethyl ester(7), 4-hydroxy-3, 5-dimethoxybenzaldehyde(8), trans-ethyl ferulate(9), ethyl 4-hydroxy-3-methoxybenzoate(10), and bis(2-ethylhexyl) phthalate(11), respectively. Conclusion: All the compounds were isolated from J. pentaneurum for the first time, and most of the isolated compounds were phenolic acids with small molecules. The results provide a basis for the efficacy basis study of J. pentaneurum.