| 多花黄精化学成分分离鉴定 |
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| 引用本文： | 宁火华,袁铭铭,邬秋萍,平欲晖,周志强,许妍,吴毅,殷海霞. 多花黄精化学成分分离鉴定[J]. 中国实验方剂学杂志, 2018, 24(22): 77-82 |
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| 作者姓名： | 宁火华 袁铭铭 邬秋萍 平欲晖 周志强 许妍 吴毅 殷海霞 |
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| 作者单位： | 江西省药品检验检测研究院江西省药品与医疗器械质量工程技术研究中心;江西中医药大学 |
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| 基金项目： | 江西省重点研发计划（C类）项目（20181BBG70046） |
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| 摘 要： | 目的:对多花黄精根茎的正丁醇和二氯甲烷部位的化学成分进行分离,并对所得化合物进行结构鉴定。方法:多花黄精的干燥根茎经江西建昌帮传统炮制方法—炆法炮制,60℃烘干,粉碎,70%乙醇浸泡1 h,加热回流提取2次,滤过,提取液合并浓缩,依次用两倍体积的二氯甲烷、乙酸乙酯、正丁醇溶液分别萃取3次,萃取液分别减压浓缩至干,得二氯甲烷萃取部分、乙酸乙酯萃取部分和正丁醇萃取部分。用大孔吸附树脂,反复硅胶柱,LH-20型羟丙基葡聚糖凝胶(Sephadex LH-20),YMC-HPLC制备柱等技术对多花黄精根茎的正丁醇和二氯甲烷部位进行分离纯化,根据化合物的理化常数测定及波谱数据分析鉴定化合物结构。结果:从多花黄精根茎的正丁醇和二氯甲烷萃取部位中分离得到14个化合物,分别鉴定为(25S)spirostan-5-en-12-one-3-O-D-glucopyranosyl-(1→2)-O-[β-D-xylopyranosyl(1→3)]-O-β-D-glucopyranosyl(1→4)-β-Dgalactopyranoside(1),(25S)spirostan-5-en-12-one-3-O-β-D-glucopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→3)]-O-β-Dglucopyranosyl-(1→4)-β-D-galactopyranoside(2),邻苯二甲酸二丁酯(3),胡萝卜苷(4),β-谷甾醇(5),hypofuran B(6),(25R)-3-β-hydroxyspirost-5-en-12-one(7),(+)-medioresinol(8),黄精碱A(9),5-羟甲基糠醛(10),5-羟基麦芽酚(11),2-(4-羟苯基)乙醇(12),1H-吲哚-3-甲醛(13),黄精碱B(14)。结论:化合物4,5,9,14为首次从多花黄精中分离得到,化合物6,8,12,13为首次从黄精属植物中分离得到。该研究为多花黄精综合开发及天然植物药理活性研究提供化学依据和物质参考。
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| 关 键 词： | 多花黄精 化学成分 正丁醇部位 二氯甲烷部位 |
| 收稿时间： | 2018-02-06 |
Identification of Chemical Constituents from Polygonatum cyrtonema |
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| NING Huo-hu,YUAN Ming-ming,WU Qiu-ping,PING Yu-hui,ZHOU Zhi-qiang,XU Yan,WU Yi and YIN Hai-xia. Identification of Chemical Constituents from Polygonatum cyrtonema[J]. China Journal of Experimental Traditional Medical Formulae, 2018, 24(22): 77-82 |
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| Authors: | NING Huo-hu YUAN Ming-ming WU Qiu-ping PING Yu-hui ZHOU Zhi-qiang XU Yan WU Yi YIN Hai-xia |
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| Affiliation: | Jiangxi Institute for Drug Control, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China,Jiangxi Institute for Drug Control, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China,Jiangxi Institute for Drug Control, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China,Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China,Jiangxi Institute for Drug Control, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China,Jiangxi Institute for Drug Control, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China,Jiangxi Institute for Drug Control, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China and Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China |
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| Abstract: | |
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| Keywords: | Polygonatum cyrtonema chemical constituents ethyl acetatefraction n-butanol fraction |
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