| UPLC-MS/MS测定何首乌中10个二苯乙烯苷类成分的含量 |
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| 引用本文： | 余意,高峰,李佳兴,金艳,马方励,杨健,郭兰萍. UPLC-MS/MS测定何首乌中10个二苯乙烯苷类成分的含量[J]. 中国实验方剂学杂志, 2018, 24(23): 87-92 |
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| 作者姓名： | 余意 高峰 李佳兴 金艳 马方励 杨健 郭兰萍 |
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| 作者单位： | 无限极(中国)有限公司;中国中医科学院西苑医院;中国中医科学院中药资源中心道地药材国家重点实验室培育基地;广东药科大学中药学院 |
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| 基金项目： | 中国中医科学院重点领域项目（ZZ10-027） |
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| 摘 要： | 目的:建立超高效液相色谱三重四级杆串联质谱法同时测定不同产地何首乌中10种二苯乙烯苷类成分的方法,比较不同产地何首乌该类成分的含量差异。方法:采用ACQUITY UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.8μm);以0.1%甲酸水(A)-0.1%甲酸乙腈(B)溶液为流动相,梯度洗脱,流速0.5 m L·min~(-1),柱温40℃;采用电喷雾离子源,负离子检测方式,得到相应的提取离子流图,以峰面积进行定量。结果:何首乌中白藜芦醇,虎杖苷,2,3,5,4'-四羟基二苯乙烯-2-O-葡萄糖苷,2,3,5,4'-四羟基二苯乙烯-2,3-O-二葡萄糖苷,2,3,5,4'-四羟基二苯乙烯-2-O-葡萄糖基-(1→6)-葡萄糖苷,2,3,5,4'-四羟基二苯乙烯-2-O-(2″-O-没食子酰基)-葡萄糖苷,2,3,5,4'-四羟基二苯乙烯-2-O-(6″-O-乙酰基)-葡萄糖苷,2,3,5,4'-四羟基二苯乙烯-2-O-(2″-O-阿魏酰基)-葡萄糖苷和2,3,5,4'-四羟基二苯乙烯-2-O-(2″-O-香豆酰基)-葡萄糖苷具有良好的线性关系,r均≥0.998 7,平均加样回收率为96.5%~106.3%,RSD均≤3.5%。结论:该方法操作简便、准确、重复性好,为何首乌二苯乙烯苷类成分的深入研究及其质量控制提供了参考依据。
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| 关 键 词： | 何首乌 二苯乙烯苷 超高效液相色谱-串联质谱法 含量测定 |
| 收稿时间： | 2018-01-02 |
Content Determination of Ten Kinds of Stilbene Glycosides in Polygoni Multiflori Radix by UPLC-MS/MS |
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| YU Yi,GAO Feng,LI Jia-xing,JIN Yan,MA Fang-li,YANG Jian and GUO Lan-ping. Content Determination of Ten Kinds of Stilbene Glycosides in Polygoni Multiflori Radix by UPLC-MS/MS[J]. China Journal of Experimental Traditional Medical Formulae, 2018, 24(23): 87-92 |
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| Authors: | YU Yi GAO Feng LI Jia-xing JIN Yan MA Fang-li YANG Jian GUO Lan-ping |
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| Affiliation: | Infinitus(China) Company Ltd., Guangzhou 510663, China,Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, China,State Key Laboratory of Dao-di Herbs Breeding Base, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,State Key Laboratory of Dao-di Herbs Breeding Base, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Infinitus(China) Company Ltd., Guangzhou 510663, China,State Key Laboratory of Dao-di Herbs Breeding Base, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China and State Key Laboratory of Dao-di Herbs Breeding Base, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | |
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| Keywords: | Polygoni Multiflori Radix stilbene glycosides UPLC-MS/MS content determination |
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