| 桂皮醛对糖尿病小鼠血糖水平的影响及机制 |
| |
| 引用本文： | 黄链莎,刘铜华,孙文,许光远,郭璇,李迎真,陈淑惠,周鹏. 桂皮醛对糖尿病小鼠血糖水平的影响及机制[J]. 中国实验方剂学杂志, 2018, 24(10): 95-100 |
| |
| 作者姓名： | 黄链莎 刘铜华 孙文 许光远 郭璇 李迎真 陈淑惠 周鹏 |
| |
| 作者单位： | 深圳市宝安区中医院;北京中医药大学教育部中医养生学重点实验室;首都医科大学附属复兴医院 |
| |
| 基金项目： | 国家自然科学基金项目（81503550）；广东省自然科学基金项目（2015A030310222） |
| |
| 摘 要： | 目的:观察桂皮醛(cinnamaldehyde,CA)降血糖作用,并探讨其作用机制。方法:6~8周龄雄性db/db小鼠24只,按血糖随机分为模型组,二甲双胍组(0.2 g·kg~(-1)),桂皮醛低剂量组(0.025 g·kg~(-1))和桂皮醛高剂量组(0.05 g·kg~(-1)),每组6只,另设同周龄C57BL/6J小鼠6只为正常组。治疗4周后,检测各组小鼠空腹血糖(fasting blood glucose,FBG),总胆固醇(total cholesterd,TC),甘油三酯(total triglyceride,TG),游离脂肪酸(free fatty acids,FFA),天门冬氨酸氨基转移酶(aspartate aminotransferase,AST),血清胰岛素(fasting insulin,Fins)水平,计算胰岛素抵抗指数(homa insulin-resistance,HOMA-IR);取肝脏组织检测肝糖原含量以及高碘酸-希夫(periodic acid-Schiff stain,PAS)染色;采用实时荧光定量聚合酶链式反应(Real-time PCR),蛋白免疫印迹法(Western blot)检测肝脏相关mRNA和蛋白表达。结果:治疗4周后,与模型组比较,CA组小鼠体质量,FBG,Fins,HOMA-IR,血脂明显降低(P0.05,P0.01),肝脏糖原含量显著升高;小鼠肝脏葡萄糖-6-磷酸酶(glucose-6-phosphate,G-6-P),磷酸烯醇式丙酮酸羧激酶(phosphoenolpyruvate carboxykinase,PEPCK)mRNA表达显著降低(P0.01),p-蛋白激酶B(protein kinase B,Akt),p-糖原合成酶激酶-3β(GSK-3β)蛋白表达显著升高(P0.01)。结论:CA具有降低血糖作用,相关机制可能是通过上调肝脏胰岛素信号通路Akt,GSK-3β磷酸化水平,抑制G-6-P,PEPCK mRNA表达实现。
|
| 关 键 词： | 桂皮醛 2型糖尿病 葡萄糖-6-磷酸酶(G-6-P)/磷酸烯醇式丙酮酸羧激酶(PEPCK) 蛋白激酶B(Akt)/糖原合成酶激酶-3β(GSK-3β) |
| 收稿时间： | 2017-08-17 |
Effect and Mechanism of Cinnamaldehyde on Insulin Resistance in db/db Mice |
| |
| HUANG Lian-sh,LIU Tong-hu,SUN Wen,XU Guang-yuan,GUO Xuan,LI Ying-zhen,CHEN Shu-hui and ZHOU Peng. Effect and Mechanism of Cinnamaldehyde on Insulin Resistance in db/db Mice[J]. China Journal of Experimental Traditional Medical Formulae, 2018, 24(10): 95-100 |
| |
| Authors: | HUANG Lian-sh LIU Tong-hu SUN Wen XU Guang-yuan GUO Xuan LI Ying-zhen CHEN Shu-hui ZHOU Peng |
| |
| Affiliation: | Baoan District Hospital of Traditional Chinese Medicine, Shenzhen 518133, China,Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, China,Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, China,Fuxing Hospital, Capital Medical University, Beijing 100045, China,Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, China,Baoan District Hospital of Traditional Chinese Medicine, Shenzhen 518133, China,Baoan District Hospital of Traditional Chinese Medicine, Shenzhen 518133, China and Baoan District Hospital of Traditional Chinese Medicine, Shenzhen 518133, China |
| |
| Abstract: | |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
|
点击此处可从《中国实验方剂学杂志》下载全文 |
|
