广东省鼠形动物携带巴尔通体的调查和基因特征分析

目的 了解巴尔通体在广东省鼠形动物中的流行分布情况及其基因型特征,为巴尔通体的研究和自然疫源性疾病的防控提供科学依据。方法 在广东省惠来县、惠东县、潮安县、罗定县和高州市5个地区进行捕鼠,采集鼠肝脏标本提取总核酸,利用聚合酶连反应(PCR)检测巴尔通体病原,并对其进行分离培养,阳性产物进行测序,并根据序列进行系统进化分析,统计分析巴尔通体的分布情况和特征。结果在广东省5个地区共捕鼠375只,检出巴尔通体阳性标本73份,阳性率为19.47%。不同鼠种(χ²=6.361)、不同地区(χ²=7.778)、不同性别(χ²=0.292)、不同生境间(χ²=0.621),阳性率差异均没有统计学意义(P>0.05)。73份样本中,10份成功分离培养出巴尔通体。系统进化分析表明广东鼠形动物携带的巴尔通体存在6种基因型:Bartonella elizabethae、 B.phoceensis、 B.japonica、 B.henselae、 B.rochalimae、B.tribocorum。结论 巴尔通体在广东省鼠形动物中广泛存在,且基因型呈多样性,其中4种基因型对人类有致病性,对人类健康具有潜在威胁。

Genotype characteristics of Bartonella and the infection in rodents in Guangdong Province,China

We investigated the distribution and genotype characteristics of Bartonella in rodents in Guangdong and provide scientific data for study of Bartonella and natural focal diseases control and prevention. Rodents were captured in five districts of Guangdong: Huilai, Huidong, Chaoan, Luoding and Gaozhou. Total nucleic acid from liver specimens was extracted and detected Bartonella using PCR. Positive simples were cultivated and the PCR products were sequenced. The phylogenetic analysis and statistical analysis were conducted. Results showed that totally 375 rodents were captured in five regions of Guangdong and 73 liver samples were positive for Batronella, the positive rate was 19.47%. There was no significant statistic differences between species(χ²=6.361),districts(χ²=7.778), gender(χ²=0.292) and habitats(χ²=0.621) (P>0.05). Of 73 specimens, 10 were positive in Bartonella culturing. The phylogenetic analysis illustrated that 6 genotypes of Bartonella were confirmed in rodents: Bartonella elizabethae, B.phoceensis, B.japonica, B.henselae, B.rochalimae, B.tribocorum. In Guangdong Province, Bartonella is widespread in rodents with obvious diverse genotypes. Four genotypes have potential hazard for human health.