| 辛芍组方中5个指标成分在大鼠体内的组织分布情况 |
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| 引用本文： | 周孟,胡贺佳,李梅,李莹,薛寸,李月婷,胡杰,黄勇,王广成,郑林,李勇军,王爱民,席晓岚,巩仔鹏.辛芍组方中5个指标成分在大鼠体内的组织分布情况[J].中国实验方剂学杂志,2018,24(19):92-98. |
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| 作者姓名： | 周孟 胡贺佳 李梅 李莹 薛寸 李月婷 胡杰 黄勇 王广成 郑林 李勇军 王爱民 席晓岚 巩仔鹏 |
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| 作者单位： | 贵州医科大学贵州省药物制剂重点实验室药用植物功效与利用国家重点实验室民族药与中药开发应用教育部工程研究中心药学院 |
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| 基金项目： | 国家自然科学基金项目（81260636）；贵州省优秀青年科技人才培养项目（黔科合平台人才[2017]5601号）；贵阳市科研创新团队项目（筑科合同[2017]30-29号）；贵州省科技厅科技创新人才团队建设项目（黔科合平台人才[2016]5613）；贵州省高层次创新型人才培养计划项目（黔科合平台人才[2016]5677） |
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| 摘 要： | 目的:研究大鼠灌胃辛芍组方后其5个指标成分在体内的组织分布情况。方法:建立同时测定大鼠组织中灯盏甲素、灯盏乙素、灯盏乙素苷元、芍药苷、芍药内酯苷的UPLC-MS/MS,将辛芍组方灌胃给予SD大鼠(剂量25 g·kg~(-1)),分别于给药后20,90 min和12,24 h取其主要脏器和组织,采用UPLC-MS/MS测定各个时间点下5个指标成分在脏器和组织中的分布情况。结果:大鼠灌胃辛芍组方后,5个指标成分可广泛分布于大鼠各组织器官中。对于灯盏甲素,其质量浓度20 min时在肺和心中达到峰值,分别为48.2,59.8μg·L~(-1);90 min时在脑和小肠中达到峰值,分别为138.7,292.7μg·L~(-1);12 h时在肾和肌肉中达到峰值,为517.3,56.5μg·L~(-1);24 h时在肝脏中达到峰值,为352.9μg·L~(-1)。对于灯盏乙素,其质量浓度20 min时在心中达到峰值,为38.3μg·L~(-1);90 min时在肺、脑、小肠和肾中达到峰值,分别为30.6,220.0,1 644.2,859.9μg·L~(-1);12 h时在肌肉中达到峰值,为86.9μg·L~(-1);24 h时在肝脏中达到峰值,为344.8μg·L~(-1)。对于灯盏乙素苷元,其质量浓度90 min时在脑、小肠和肌肉中达到峰值,分别为875.4,52 468.0,5 114.7μg·L~(-1);12 h时在肺、心和肾中达到峰值,分别为609.7,1 718.6,1 736.5μg·L~(-1);24 h时在肝脏中达到峰值,为1 366.5μg·L~(-1)。对于芍药苷,其质量浓度20 min时在肺、肝、肾和肌肉中达到峰值,分别为10 634.1,13 889.0,16 672.0,7 750.4μg·L~(-1);在90 min时,在心、小肠和脑中达到峰值,分别为6 851.1,870 563.9,7 474.0μg·L~(-1)。对于芍药内酯苷,其质量浓度20 min时在肝和肾中达到峰值,分别为15 249.7,56 817.0μg·L~(-1),在90 min时,在肺、心、脑、小肠和肌肉中达到峰值,分别为4 211.5,4 425.3,4 631.3,631 871.5,5 673.6μg·L~(-1)。结论:大鼠灌胃辛芍组方后,5个指标成分可迅速、广泛地分布在各组织器官中,但5个指标成分在各组织脏器中的达峰时间和分布存在一定差异,在肾脏和小肠中的分布最高,其次为肝和肌肉,同时在脑组织中也有分布,并且随着给药时间的延长,灯盏甲素、灯盏乙素和灯盏乙素苷元可能在大鼠的肝脏中会发生蓄积。
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| 关 键 词： | 辛芍组方 灯盏乙素 灯盏甲素 芍药苷 组织分布 |
| 收稿时间： | 2018/4/18 0:00:00 |
Tissue Distribution of Five Components from Xinshao Formula in Rats |
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| ZHOU Meng,HU He-ji,LI Mei,LI Ying,XUE Cun,LI Yue-ting,HU Jie,HUANG Yong,WANG Guang-cheng,ZHENG Lin,LI Yong-jun,WANG Ai-min,XI Xiao-lan and GONG Zi-peng.Tissue Distribution of Five Components from Xinshao Formula in Rats[J].China Journal of Experimental Traditional Medical Formulae,2018,24(19):92-98. |
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| Authors: | ZHOU Meng HU He-ji LI Mei LI Ying XUE Cun LI Yue-ting HU Jie HUANG Yong WANG Guang-cheng ZHENG Lin LI Yong-jun WANG Ai-min XI Xiao-lan and GONG Zi-peng |
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| Institution: | Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China,Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China and Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China |
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| Abstract: | |
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| Keywords: | Xinshao formula scutellarin apigenin-7-O-glucronide paeoniflorin tissue distribution |
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