壮骨伸筋胶囊对去卵巢大鼠骨质疏松症的治疗及其作用机制

目的:研究壮骨伸筋胶囊(Zhuanggu Shenjin capsule,ZGSJC)对去卵巢骨质疏松大鼠的治疗作用,探讨ZGSJC治疗绝经后骨质疏松的机制。方法:将去卵巢大鼠随即分为模型组,阳性药结合雌激素组,ZGSJC高、中、低剂量组(生药5.42,2.71,1.36 g·kg-1),另设假手术组,每组12只。实验结束,取右侧胫骨制作不脱钙骨切片,用于骨形态计量学考察;左侧胫骨提取蛋白后用蛋白免疫印迹法测定Ⅰ型胶原蛋白表达。结果:与假手术比,模型组大鼠骨小梁体积百分率(TBV)显著降低(P0.01),骨小梁吸收表面百分率(TRS),骨小梁形成表面百分率(TFS),骨小梁矿化率(MAR),类骨质平均宽度(OSW),骨皮质矿化率(m AR)显著增高(P0.01),Ⅰ型胶原蛋白表达显著降低(P0.01);与模型组比,ZGSJC能够显著抑制TBV%的降低(P0.01),抑制TRS,TFS,MAR,m AR的升高(P0.01,P0.05),提高Ⅰ型胶原蛋白表达(P0.01),对OSW则无明显作用。结论:壮骨伸筋胶囊对卵巢切除所致大鼠骨质疏松具有一定的治疗作用,具体体现在调节去卵巢所致的骨代谢高转换状态,能提高Ⅰ型胶原蛋白表达含量。

Effect and Mechanism of Zhuanggu Shenjin Capsule in Ovariectomized Osteoporosis Rats

Objective: To investigate the effects of Zhuanggu Shenjin capsule (ZGSJC)on bone histomorphometry and the expression of type I collagen protein in ovariectomized rats(OVX). And then to explore the mechanism of treating osteoporosis with ZGSJC. Method: 72 female SD rata were randomly divided into 6 groups after ovariectomy:sham group, model group, positive control group and ZGSJC treatment group with doses of 5.42, 2.71, 1.36 g·kg^-1. Each group included 12 rats. After treated 3 moths, right tibia were dissected and undecalcified bone slice were made for bone histomorphometry detection, percentage of trabecular volume(TBV),bone trabecular absorption surface percentage(TRS),Bone trabecular surface percentage(TFS),average osteoid width(OSW),bone trabecular mineralization rate(MAR),bone cortical mineralization rate(mAR) were recorded. Used left tibia to extract protein and the expression of type I collagen protein was analyzed by Western blot. Result: In contrast with sham rats, OVX rats showed significant decreased in tibia TVB%, significant increase in tibia TRS%, TFS%, MAR, OSW and mAR. And showed significant decrease in type I collagen protein expression; compared with model rats, ZGSJC can inhibit the decrease of TVB% in tibia, inhibit the increase of TRS%, TFS%, MAR, OSW and mAR in tibia and inhibit the decrease expression of type I collagen protein. Conclusion: ZGSJC has the therapeutic effect in OVX Osteoporosis which could regulate bone great rate of transformation in OVX Osteoporosis; ZGSJC can increase the expression of type I collagen protein,which are the mechanisms that ZGSJC can be treated for Osteoporosis.