| 辣蓼对大肠埃希菌性腹泻小鼠肠黏膜的修复作用 |
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| 引用本文： | 肖祝华,罗晓韵,程轩轩,郑诗嘉,杨全,杨慧文. 辣蓼对大肠埃希菌性腹泻小鼠肠黏膜的修复作用[J]. 中国实验方剂学杂志, 2018, 24(22): 120-126 |
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| 作者姓名： | 肖祝华 罗晓韵 程轩轩 郑诗嘉 杨全 杨慧文 |
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| 作者单位： | 广东药科大学中药学院国家中医药管理局岭南药材生产与开发重点研究室国家中药材产业技术体系广州综合试验站广东省南药规范化种植与综合开发工程技术研究中心 |
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| 基金项目： | 广东省公益研究与能力建设专项项目（2014A020208133） |
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| 摘 要： | 目的:研究辣蓼对大肠埃希菌性腹泻小鼠的干预作用,并探讨其可能的作用机制。方法:选取160只昆明种小鼠随机分为正常组,模型组,小檗碱组,辣蓼低、中、高剂量组。除正常组外,其余各组采用腹腔注射致病性大肠埃希菌建立小鼠腹泻模型。辣蓼低、中、高剂量组分别灌胃2.5,5,7.5 g·kg-1辣蓼水提液,小檗碱组给予盐酸小檗碱0.025 g·kg-1,正常组及模型组以等体积生理盐水,每天给药2次。分别于成模后的第1,2,4,6天各组随机抽取5只动物,取十二指肠,光镜下观察病理形态学变化;采用酶联免疫吸附法(ELISA)测定肠组织中炎症因子白细胞介素-1β(interleukin-1β,IL-1β)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平;实时荧光定量聚合酶链式反应(Real-time PCR)检测肠组织中转化生长因子-β1(transforming growth factor-β1,TGF-β1),表皮生长因子受体(epidermal growth factor receptor,EGFR),Smad3及Smad7 mRNA表达水平。结果:病理学观察发现辣蓼干预后可以明显减轻腹泻小鼠十二指肠黏膜损伤;ELISA结果表明,与正常组比较,模型组小鼠肠黏膜TNF-α和IL-1β的含量明显增多(P0.01);与模型组比较,辣蓼中、高剂量组能降低小鼠肠黏膜TNF-α和IL-1β的含量(P0.05,P0.01)。Real-time PCR结果表明,与正常组比较,模型组TGF-β1,EGFR表达量均明显升高(P0.05,P0.01),Smad7 mRNA表达量明显降低(P0.05,P0.01),药物干预后,与模型组比较,各给药组可不同程度调节肠组织中TGF-β1,EGFR,Smad3及Smad7 mRNA表达量(P0.05),且呈现一定的剂量依赖关系。结论:辣蓼对大肠埃希菌性腹泻有良好的治疗作用,其作用机制可能与减少肠组织炎症因子释放、改善肠道黏膜屏障、干预TGF-β/Smads信号转导有关。
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| 关 键 词： | 辣蓼 大肠埃希菌性腹泻 肿瘤坏死因子-α(TNF-α) 白细胞介素-1β(IL-1β) 转化生长因子-β1(TGF-β1) 表皮生长因子受体(EGFR) TGF-β/Smads信号通路 |
| 收稿时间： | 2018-04-24 |
Effect of Polygoni Hydropiperis Herba on Intestinal Mucosal Repair in Diarrhea Mice Induced by Escherichia coli |
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| XIAO Zhu-hu,LUO Xiao-yun,CHENG Xuan-xuan,ZHENG Shi-ji,YANG Quan and YANG Hui-wen. Effect of Polygoni Hydropiperis Herba on Intestinal Mucosal Repair in Diarrhea Mice Induced by Escherichia coli[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(22): 120-126 |
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| Authors: | XIAO Zhu-hu LUO Xiao-yun CHENG Xuan-xuan ZHENG Shi-ji YANG Quan YANG Hui-wen |
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| Affiliation: | Key Laboratory of State Administration of Traditional Chinese Medicine(TCM) for Production & Development of Cantonese Medicinal Materials, Comprehensive Experimental Station of Guangzhou, Chinese Materia Medica, China Agriculture Research System, Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China,Key Laboratory of State Administration of Traditional Chinese Medicine(TCM) for Production & Development of Cantonese Medicinal Materials, Comprehensive Experimental Station of Guangzhou, Chinese Materia Medica, China Agriculture Research System, Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China,Key Laboratory of State Administration of Traditional Chinese Medicine(TCM) for Production & Development of Cantonese Medicinal Materials, Comprehensive Experimental Station of Guangzhou, Chinese Materia Medica, China Agriculture Research System, Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China,Key Laboratory of State Administration of Traditional Chinese Medicine(TCM) for Production & Development of Cantonese Medicinal Materials, Comprehensive Experimental Station of Guangzhou, Chinese Materia Medica, China Agriculture Research System, Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China,Key Laboratory of State Administration of Traditional Chinese Medicine(TCM) for Production & Development of Cantonese Medicinal Materials, Comprehensive Experimental Station of Guangzhou, Chinese Materia Medica, China Agriculture Research System, Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China and Key Laboratory of State Administration of Traditional Chinese Medicine(TCM) for Production & Development of Cantonese Medicinal Materials, Comprehensive Experimental Station of Guangzhou, Chinese Materia Medica, China Agriculture Research System, Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China |
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