金蓝颗粒对病毒感染幼年动物致急性咽炎模型的影响

目的:观察金蓝颗粒对柯萨奇病毒、腺病毒、流感病毒感染致幼年白兔、小鼠急性咽炎模型的影响。方法:取幼年白兔48只,按体质量随机分为8组,分别为正常组,模型组,利巴韦林组(22.5 mg·kg-1·d-1),小儿清咽组(2.7 g·kg-1·d-1),金蓝颗粒2倍剂量组、等倍剂量组、1/2倍剂量组及1/3倍剂量组(2.18,1.09,0.55,0.36 g·kg-1·d-1),每组6只。采用柯萨奇B4病毒感染致幼年白兔急性咽炎模型,金蓝颗粒连续灌胃给药3 d后,取咽部组织进行病理学检查;采用腺病毒连续感染3次致幼年小鼠急性咽炎模型,金蓝颗粒连续给药3 d后,取咽部黏膜进行病变观察;采用流感病毒FM1株感染幼年小鼠后,观察感染后2周内动物的死亡情况。结果:与正常组比较,模型组柯萨奇病毒感染幼年白兔后,咽部黏膜病变明显;与模型组比较,金蓝颗粒4个剂量组均能明显减轻咽部黏膜的病变程度。与正常组比较,模型组腺病毒感染幼年小鼠后,咽部黏膜病变明显;与模型组比较,金蓝颗粒4个剂量组均能明显减轻咽部黏膜的病变程度;结论:金蓝颗粒对柯萨奇病毒、腺病毒感染幼年白兔、小鼠导致的急性咽炎具有明显的抑制作用。

Effect of Jinlan Granule on Acute Pharyngitis Induced by Virus Infection in Infancy Animals

Objective: To observe the effect of Jinlan granule on acute pharyngitis induced by Coxsackie virus, adenovirus, and influenza virus infection in infancy rabbits and infancy mice. Method: Totally 48 infancy rabbits were randomly divided into normal group, model group, Ribavirin group (22.5 mg·kg^-1·d^-1), Xiaoer Qingyan group (2.7 g·kg^-1·d^-1), Jinlan granule double dose group, equal dose group, 1/2 dose group, and 1/3 dose group (2.18, 1.09, 0.55, 0.36 g·kg^-1·d^-1), n=6 in each group. Acute pharyngitis model in infancy rabbits was induced by Coxsackie B4 virus infection, and then Jinlan granule was successively administered daily for 3 days beginning from the day of infection. After that, pharynx tissue was taken for pathological examination. Infancy mice were infected by adenovirus successively for 3 times to induce acute pharyngitis model in infancy mice, and then Jinlan granule was successively administered daily for 3 days beginning from the day of infection. After that, pharyngeal mucosa was taken to observe pathological changes. After infancy mice were infected by influenza virus, the number of mice death in the 2 weeks after infection was observed. Result: As compared with normal group, pharyngeal tissues of rabbits showed obvious pathological changes after infection with coxsackie virus; as compared with the model group, Jinlan granule at 4 different doses could significantly relieve the pathological changes of pharyngeal tissues. As compared with the normal group, pharyngeal tissues of mice showed obvious pathological changes after infection with adenovirus, and were significantly relieved by Jinlan granule at doses of 6.48, 3.24, 1.62, 1.08 g·kg^-1·d^-1. Conclusion: Jinlan granule has significant inhibitory effect on acute pharyngitis induced by coxsackie virus and adenovirus in infancy rabbits and mice respectively.