| 五味子不同部位黄酮和酚酸类成分的含量测定及抗氧化活性 |
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| 引用本文： | 金银萍,曲正义,崔丽丽,朴向民,郭靖,王英平.五味子不同部位黄酮和酚酸类成分的含量测定及抗氧化活性[J].中国实验方剂学杂志,2018,24(19):79-84. |
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| 作者姓名： | 金银萍 曲正义 崔丽丽 朴向民 郭靖 王英平 |
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| 作者单位： | 中国农业科学院特产研究所 |
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| 基金项目： | 吉林省科技厅科技成果转化项目（20170309012YY）；吉林省科技发展计划项目（201603083YY） |
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| 摘 要： | 目的:建立超高效液相色谱法测定五味子不同部位中黄酮和酚酸类成分的含量,并研究其体外的抗氧化活性。方法:运用ACQUITY UPLC BEH C18色谱柱(2.1 mm×5.0 mm,1.7μm),乙腈-0.01磷酸水梯度洗脱,柱温30℃。黄酮类成分检测波长为360 nm,流速0.35 m L·min~(-1);酚酸类成分检测波长为280 nm,流速0.30 m L·min~(-1)。以DPPH和ABTS法对其抗氧化活性进行比较。结果:五味子不同部位中黄酮和酚酸类成分含量差异显著,叶中黄酮类成分的含量,约为藤茎黄酮类成分含量的3.8倍,果实的49.5倍;叶中酚酸类成分的含量,约为藤茎酚酸类成分含量的4.2倍,果实的21.5倍。叶、藤茎的抗氧化活性显著高于果实,叶、藤茎的DPPH自由基清除率分别为果实的8.3倍和4.3倍;ABTS总抗氧化能力分别为果实的2.9倍和1.3倍。结论:五味子不同部位中总黄酮和酚酸成分含量和抗氧化活性具有显著正相关,表明五味子抗氧化活性可能与总黄酮和酚酸类物质的含量有关。该方法简便易行,结果准确,重复性好,可用于五味子药材中黄酮和酚酸类成分的含量测定,同为五味子不同部位的合理应用提供一定的理论依据。
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| 关 键 词： | 五味子 黄酮 酚酸 抗氧化 |
| 收稿时间： | 2017/12/11 0:00:00 |
Quantification of Flavonoids and Phenols and Their Antioxidant Activities in Different Parts of Schisandra chinensis |
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| JIN Yin-ping,QU Zheng-yi,CUI Li-li,PIAO Xiang-min,GUO Jing and WANG Ying-ping.Quantification of Flavonoids and Phenols and Their Antioxidant Activities in Different Parts of Schisandra chinensis[J].China Journal of Experimental Traditional Medical Formulae,2018,24(19):79-84. |
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| Authors: | JIN Yin-ping QU Zheng-yi CUI Li-li PIAO Xiang-min GUO Jing and WANG Ying-ping |
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| Institution: | Institute of Special Animal and Plant Science of Chinese Academy of Agricultural Sciences, Changchun 130112, China,Institute of Special Animal and Plant Science of Chinese Academy of Agricultural Sciences, Changchun 130112, China,Institute of Special Animal and Plant Science of Chinese Academy of Agricultural Sciences, Changchun 130112, China,Institute of Special Animal and Plant Science of Chinese Academy of Agricultural Sciences, Changchun 130112, China,Institute of Special Animal and Plant Science of Chinese Academy of Agricultural Sciences, Changchun 130112, China and Institute of Special Animal and Plant Science of Chinese Academy of Agricultural Sciences, Changchun 130112, China |
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| Abstract: | |
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| Keywords: | Schisandra chinensis flavonoid phenol antioxidant activity |
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