路邓葡萄球菌的鉴定、耐药和临床感染分析

目的探讨路邓葡萄球菌临床分布特点及其耐药情况。 方法收集来自解放军第261医院和北京市海淀医院共34株菌株，采用VITEK-2 Compact和MALDI-TOF MS蛋白质谱进行路邓葡萄球菌的鉴定，使用革兰阳性菌细菌药敏卡片VITEK-2 AST-GP67 Test Kit试剂盒和WHONET 5.6进行药敏试验分析，采用Fisher确切概率法进行统计分析。 结果34株临床分离的路邓葡萄球菌中，皮肤软组织感染伤口分泌物占首位（47%），其次为骨和关节感染、乳腺疾病和妇科宫颈疾病共占9%，再次为血流感染、耳部疾病、鼻部疾病和泌尿系统感染共占6%；路邓葡萄球菌除对青霉素耐药率达到91%以外，对大部分抗菌药物敏感，耐甲氧西林路邓葡萄球菌阳性菌株比例为32%，阳性组和阴性组对抗菌药物耐药率差异无统计学意义（P均> 0.05）。 结论路邓葡萄球菌所致感染部位主要在皮肤软组织、乳腺、骨和关节部位、血液、耳鼻部和泌尿生殖部位，且对临床大部分抗菌药物比较敏感。

Identification,drug resistance and clinical infection analysis of Staphylococcus lugdunensis

ObjectiveTo investigate the clinical distribution and the drug resistance of Staphylococcus lugdunensis. MethodsTotal of 34 strains from the 261^st Hospital of the People’s Liberation Army and Haidian Hospital of Beijng were collected. Staphylococcus lugdunensis were identified by VITEK-2 Compact and MALDI-TOF MS apply protein mass spectrometry. The drug resistance was analyzed by Gram-positive bacteria susceptibility test product VITEK-2 AST-GP67 Test Kit and WHONET 5.6 and the statistical analysis were performed by Fisher exact probabilities. ResultsWound secretion due to skin and soft tissue infections, accounting for 47%; following by bone and joint infections, breast diseases and gynecological diseases, account for 9%, then following by bloodstream infections, ear problems, nose diseases and urinary system infections, account for 6%. The resistance of Staphylococcus lugdunensis to penicillin reached 91%, but it was sensitive to most other antibacterial medicines. Positive bacteria strains of Methicillin-resistant Staphylococcus lugdunensis account for 32%. There was no significant difference of the resistant rates to antibacterial drugs between patients in the positive group and negative group. ConclusionsThe infection sites of Staphylococcus lugdunensis were mainly in skin, tissues, breast bone, joint, none, ears and genitourinary region, and were sensitive to most of the clinical antibiotics.