| HPLC-MS测定小儿安神补脑颗粒中8个成分的含量 |
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| 引用本文： | 高厚明,胡兆流,郑平,李中桂,郑琳,邱静宇,易铁钢,张尚斌,陈剑平.HPLC-MS测定小儿安神补脑颗粒中8个成分的含量[J].中国实验方剂学杂志,2018,24(19):73-78. |
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| 作者姓名： | 高厚明 胡兆流 郑平 李中桂 郑琳 邱静宇 易铁钢 张尚斌 陈剑平 |
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| 作者单位： | 深圳市中医院深圳市医院中药制剂研究重点实验室 |
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| 基金项目： | 深圳市科技计划项目（JCYJ20150401163247216，ZDSYS201606081515458）；广东省中医药局项目（20151079）；中华人民共和国人力资源和社会保障部留学人员科技活动项目（人社厅函[2015]192号）） |
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| 摘 要： | 目的:建立高效液相色谱-串联质谱法同时测定小儿安神补脑颗粒中8个成分的含量测定方法。方法:采用Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈(A)-10 mol·L~(-1)乙酸铵(B)水溶液,梯度洗脱;进样量10μL。质谱条件采用电喷雾离子源,正负离子同时检测,扫描方式为选择离子扫描模式。结果:甘草苷,毛蕊异黄酮葡萄糖苷,甘草酸铵,柚皮苷,3,6'-二芥子酰基蔗糖,橙皮苷,新橙皮苷和黄芪甲苷线性范围分别在0.019 5~2.5 mg·L~(-1)(r=0.999 1),0.078 1~5 mg·L~(-1)(r=0.999 2),0.078 1~5 mg·L~(-1)(r=0.999 8),0.312 5~50 mg·L~(-1)(r=0.999 9),0.019 5~2.5 mg·L~(-1)(r=0.999 2),0.078 1~10 mg·L~(-1)(r=0.999 0),0.312 5~20 mg·L~(-1)(r=0.999 4),0.019 5~1.25 mg·L~(-1)(r=0.999 0);精密度、稳定性、重复性试验的RSD均2%;平均回收率在95.07%~97.79%,RSD均2%;甘草苷,毛蕊异黄酮葡萄糖苷,甘草酸铵,柚皮苷,3,6'-二芥子酰基蔗糖,橙皮苷,新橙皮苷和黄芪甲苷样品含量测定结果依次为187.5~188.1,330.0~331.0,312.1~319.5,1 509.0~1 510.2,172.2~173.0,180.0~181.0,639.0~639.8,37.5~38.2μg·g-1。结论:该方法简单、准确、灵敏、重复性好,可用于小儿安神补脑颗粒的质量评价与控制。
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| 关 键 词： | 小儿安神补脑颗粒 甘草苷 毛蕊异黄酮葡萄糖苷 甘草酸铵 柚皮苷 3 6''-二芥子酰基蔗糖 橙皮苷 新橙皮苷 黄芪甲苷 |
| 收稿时间： | 2018/2/5 0:00:00 |
Simultaneous Determination of Eight Components in Xiaoer Anshen Bunao Granule by HPLC-MS |
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| GAO Hou-ming,HU Zhao-liu,ZHENG Ping,LI Zhong-gui,ZHENG Lin,QIU Jing-yu,YI Tie-gang,ZHANG Shang-bin and CHEN Jian-ping.Simultaneous Determination of Eight Components in Xiaoer Anshen Bunao Granule by HPLC-MS[J].China Journal of Experimental Traditional Medical Formulae,2018,24(19):73-78. |
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| Authors: | GAO Hou-ming HU Zhao-liu ZHENG Ping LI Zhong-gui ZHENG Lin QIU Jing-yu YI Tie-gang ZHANG Shang-bin and CHEN Jian-ping |
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| Institution: | Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China,Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China,Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China,Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China,Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China,Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China,Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China,Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China and Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China |
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