不同炮制加工的熟地黄对雌性大鼠排卵功能的影响

目的:探究不同炮制加工的熟地黄水煎液对雌性大鼠排卵功能的影响,为熟地黄的炮制加工与临床用药提供参考。方法:分别采用清蒸法(一蒸一晒)和传统法(九蒸九晒)炮制生地黄,得到2种不同炮制工艺的熟地黄。以动情周期正常的雌性成年大鼠为研究对象,设空白组和清蒸组、传统组。观察这2种熟地黄的水煎液对雌性大鼠动情周期、卵巢组织形态及血清激素水平的影响,清蒸组和传统组的给药剂量均为3.6 g·kg-1。建立熟地黄的HPLC指纹图谱,并结合药效学指标研究熟地黄对雌性大鼠排卵功能影响的差异。结果:与空白组比较,清蒸组大鼠血清中雌二醇和孕酮含量明显降低(P0.05),动情周期明显延长(P0.05),卵巢中闭锁卵泡数目明显升高(P0.05);传统组各项指标变化皆不明显,与空白组相比无统计学差异。谱效学结果显示毛蕊花糖苷与清蒸法制备的熟地黄抑制大鼠排卵作用呈正相关;传统法制备的熟地黄HPLC图谱中3,5号峰与抑制排卵作用呈负相关。结论:清蒸法炮制的熟地黄对正常雌性大鼠的排卵有一定抑制作用,传统法炮制的熟地黄在排卵功能方面有一定优势。

Effect of Rehmanniae Radix Praeparata Processed with Different Techniques on Ovulation Function of Female Rats

Objective: To explore the influence of Rehmanniae Radix Praeparata decoction processed with different techniques on the ovulation function of female rats,and to provide reference for the processing and clinical use of this decoction pieces. Method: Steamed method (one steaming and one drying) and traditional method (nine steaming and nine drying) were used to prepared Rehmanniae Radix Praeparata,respectively.Female adult rats with a normal estrus cycle were selected as study subjects,blank group,steaming group and traditional group were set up.The effects of two Rehmanniae Radix Praeparata decoctions on the estrous cycle,ovarian tissue morphology and serum hormone levels in female rats were observed.The doses in the steaming group and the traditional group were 3.6 g·kg^-1.The HPLC fingerprints of Rehmanniae Radix Praeparata was established,fingerprints and pharmacodynamic indexes were used to investigate effect of Rehmanniae Radix Praeparata on ovulation of female rats. Result: Compared with the blank group,serum estradiol and progesterone levels in the steaming group were significantly decreased(P<0.05),the estrous cycle was significantly prolonged(P<0.05),and the number of atresia follicles in the ovary was significantly increased(P<0.05);the change of each index in the traditional group was not obvious.Verbascoside and effect of inhibiting ovulation of Rehmanniae Radix Praeparata processed steamed method in rats positively correlated;peak 3 and 5 in HPLC fingerprints of Rehmanniae Radix Praeparata processed with traditional method were negatively correlated with inhibition of ovulation. Conclusion: Rehmanniae Radix Praeparata processed by steamed method has a certain inhibitory effect on ovulation of normal female rats.Rehmanniae Radix Praeparata processed by traditional method has some advantages in ovulation function.