| UPLC-MS/MS分析不同品种蓝莓中的花青苷 |
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| 引用本文： | 刘彩芬,秦公伟,韩豪,李新生,刘旭,赵保承,李奔. UPLC-MS/MS分析不同品种蓝莓中的花青苷[J]. 中国实验方剂学杂志, 2018, 24(21): 62-68 |
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| 作者姓名： | 刘彩芬 秦公伟 韩豪 李新生 刘旭 赵保承 李奔 |
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| 作者单位： | 陕西理工大学生物科学与工程学院;陕西省资源生物重点实验室;陕南秦巴山区生物资源综合开发利用协同创新中心 |
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| 基金项目： | 陕西省教育厅项目（17JK0141）;陕西省科技厅科技统筹创新项目（2016KTCQ02-07） |
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| 摘 要： | 目的:采用超高效液相色谱-三重四级杆串联质谱联用技术(UPLC-MS/MS)分析12个品种蓝莓中的花青苷。方法:80%乙醇作为溶剂超声辅助提取蓝莓花青苷,提取液经离心后的上清液于旋转蒸发仪浓缩用6%甲酸水溶液定容至刻度线,超滤后,采用UPLC-MS/MS,ACQUITY UPLC BEHC18色谱柱(2.1 mm×50 mm,1.7μm),乙腈-6%甲酸水溶液作为流动相,进行梯度洗脱,在电喷雾离子源(ESI)正离子模式下,用MRM模式监测。结果:12个品种蓝莓中花青苷种类较为相似,花青苷种类最丰富的蓝莓品种是R26,含有23种,花青苷种类最少的蓝莓品种是N15,含有20种,飞燕草素和锦葵素是蓝莓中最主要的两大类花青苷。12个品种蓝莓中总花青苷含量在820.43~2 114.627μg·g-1,存在显著差异(P0.05),由大到小依次为R26N12S28R6S30S7-1S12S7-2S7-3N15N1N14。R系列总花青苷含量高于S系列,N系列花青苷含量最少。不同品种蓝莓中各类花青苷含量亦存在显著差异。结论:12个品种蓝莓中的总花青苷含量存在显著差异,可为以蓝莓花青苷为功能成分的保健品、药物开发提供基础数据,同时也为蓝莓花青苷活性实验、蓝莓花青苷的检测分析提供参考依据。
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| 关 键 词： | 超高效液相色谱-三重四级杆串联质谱 蓝莓 花青苷 鉴定 |
| 收稿时间： | 2018-04-22 |
Analysis of Anthocyanins in Different Blueberry Cultivars by UPLC-MS/MS |
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| LIU Cai-fen,QIN Gong-wei,HAN Hao,LI Xin-sheng,LIU Xu,ZHAO Bao-cheng and LI Ben. Analysis of Anthocyanins in Different Blueberry Cultivars by UPLC-MS/MS[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(21): 62-68 |
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| Authors: | LIU Cai-fen QIN Gong-wei HAN Hao LI Xin-sheng LIU Xu ZHAO Bao-cheng LI Ben |
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| Affiliation: | College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China,College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China;Shaanxi Key Laboratory of Bio-Resource, Hanzhong 723000, China,College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China;Shaanxi Key Laboratory of Bio-Resource, Hanzhong 723000, China,College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China;Shaanxi Key Laboratory of Bio-Resource, Hanzhong 723000, China;Comprehensive Development and Utilization Collaborative Innovation Center of Biological Resources in Qinba Mountain Area of Shaanxi Province, Hanzhong 723000,College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China,College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China and College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China |
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| Abstract: | |
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| Keywords: | UPLC-MS/MS blueberry anthocyanins identification |
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