基于网络药理学预测银杏蜜环口服溶液治疗冠心病与焦虑症的分子机制

目的:对银杏蜜环口服溶液进行网络药理学研究,探究其治疗冠心病与焦虑症的分子作用机制。方法:通过比较毒理基因组学数据库(Comparative Toxicogenomics Database,CTD)数据库获得银杏蜜环口服溶液有效成分和通过人类表型本体(HPO)数据库和Disease Connect数据库获得冠心病、焦虑症的靶点基因,运用STRING数据库,构建银杏蜜环口服溶液有效成分和冠心病,焦虑症的靶点基因网络。通过DAVID数据库综合比较分析,获得银杏蜜环口服溶液治疗冠心病,焦虑症的靶点基因的基因本体(Gene_Ontology,GO)功能和生物学通路。结果:银杏蜜环口服溶液的靶点基因网络有磷酸酶与张力蛋白同源物(PTEN),白细胞介素-10(IL-10),Smad3等32个基因,557个GO功能如生物质量调控、脂质稳态、睡眠的恒定机制等,20条京都基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)与冠心病重叠;有死骨片蛋白1(SQSTM1),超氧化物歧化酶1(SOD1),对氧磷酶1(PON1)等16个基因,260个GO功能如发育过程、细胞成分组织、声音的感官知觉等,10条KEGG通路与焦虑症重叠。经文献验证有10条通路和冠心病相关,4条通路与焦虑症有关。结论:银杏蜜环口服溶液可能通过干预MAPK信号通路同时对冠心病和焦虑症起到治疗效果。

Molecular Mechanisms of Yinxing Mihuan Oral Solution on Coronary Heart Disease and Anxiety Disorder Based on Network Pharmacology

Objective: To reveal the molecular mechanism of Yinxing Mihuan oral solution for coronary heart disease and anxiety. Method: The effective ingredients in Yinxing Mihuan oral solution were obtained from Comparative Toxicogenomics Database (CTD), and thetarget genes of coronary heart disease and anxiety were obtained from Human Phenotype Ontology(HPO) and Disease Connect.STRING database was used to build thetarget gene networks of the effective components in Yinxing Mihuan oral solution and coronary heart disease and anxiety. The Gene_Ontology(GO)biological process functions and targeted Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways of the gene networks of the drug and the two diseases were compared by DAVID bioinformatic software 6.7. Result: The target gene network of Yinxing Mihuan oral solution had 32 genes, such as phos-phatase and tensin homolog(PTEN), interleukin(IL)-10, Smad3, 557 GO functions, such asregulation of biological, qualitylipid homeostasis and homeostatic process; 20 KEGG pathways were overlapped with coronary heart disease; 16 genes, such as sequestosome 1(SQSTM1), super oxide dismutase(SOD1), paraoxyphosphatase 1(PON1), 260 GO functions, such as developmental process, cellular component organization, sensory perception of sound, and 10 KEGG pathways were overlapped with anxiety disorders.Literatures verified that 10 pathways were associated with coronary heart disease and 4 pathways were associated with anxiety. Conclusion: Yinxing Mihuan oral solution may play a therapeutic effect on coronary heart disease and anxiety by intervening MAPK signaling pathway.