小续命汤对急性脑缺血再灌注损伤BDNF,GDNF表达的影响

目的:观察小续命汤对大鼠急性脑缺血再灌注损伤后脑源性神经营养因子(brain derived neurotrophic factor,BDNF),胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)表达的影响。方法:60只雄性SPF级SD大鼠随机分为假手术组、模型组、小续命汤低、中、高(15,30,60 g·kg~(-1))剂量组。各组大鼠于造模前3 d开始给药直至观察节点结束,采用大脑中动脉线栓法建立脑缺血再灌注损伤模型,采用苏木素-伊红(HE)染色,Nissl染色观察脑缺血再灌注损伤,采用蛋白免疫印迹法(Western blot)和免疫组织化学法检测脑缺血再灌注损伤后的BDNF,GDNF蛋白表达。结果:与模型组比较,小续命汤高、中剂量组可显著改善其神经功能缺损(P0.05),减轻脑缺血再灌注引起的缺血性损伤;小续命汤高、中剂量组还可显著上调BDNF,GDNF蛋白表达(P0.05)。结论:小续命汤可能通过上调脑缺血再灌注后缺血半暗带区BDNF,GDNF的表达发挥脑保护的作用。

Effect of Xiao Xumingtang on Expression of BDNF and GDNF in Acute Cerebral Ischemia Reperfusion Injury

Objective: To observe the effects of Xiao Xumingtang (XXMT) on brain derived neurotrophic factor (BDNF)and glial cellderived neurotrophic factor (GDNF)expression levels after acute cerebral ischemia reperfusion injury in rats. Method: A total of 60 male Sprague-Dawley rats were randomly divided into 5 groups:sham operation group (Sham), model group (IR), and XXMT low dose, middle dose and high dose groups (15, 30, 60 g·kg^-1·d^-1). The Drugs were given from 3 days efore modeling to the end of observation nodes. Cerebral ischemia reperfusion injury models were established by using middle cerebral artery occlusion. Then the pathological changes were estimated by hematoxylin-eosin (HE) staining and Nissl staining at 24 h after reperfusion. BDNF and GDNF expression levels after cerebral ischemia reperfusion injury were quantitatively determined by Western blot and immunohistochemistry. Result: The results indicated that XXMT middle and high dose (30, 60 g·kg^-1·d^-1) treatment significantly improved neurologic impairment (P<0.05), reduced ischemic injury, and up-regulated the expression levels of BDNF and GDNF as compared with IR group(P<0.05). Conclusion: These findings indicated that XXMT treatment may exert the neuroprotective effect on cerebral ischemia by up-regulating expression levels of BDNF and GDNF.