化痰消瘤方对荷Lewis肺癌小鼠nm-23,VEGF表达的影响

目的:观察化痰消瘤方对Lewis肺癌小鼠瘤质量及肺转移灶的影响,检测瘤组织中血管内皮生长因子(VEGF),nm-23 mRNA表达的情况,探究其作用机制。方法:取雄性C57BL/6J小鼠30只,随机分为空白组,化痰消瘤方组(50 g·kg~(-1)·d~(-1)),环磷酰胺(CTX)组[0.06 g·kg~(-1)·(3,7 d)~(-1)],共3组,每组10只。右腋下注射细胞悬液建立荷Lewis肺癌小鼠模型,造模后观察21 d,处死,剥离瘤组织,计算抑瘤率,摘取肺组织,固定后显微镜下观察肺转移灶个数,计算抗肺转移率,通过实时荧光定量PCR(Real-time PCR)检测瘤组织中nm-23,VEGF mRNA表达情况,蛋白免疫印迹法(Western blot)检测瘤组织中nm-23,VEGF蛋白的表达情况。结果:与空白组比较,化痰消瘤方组及环磷酰胺组瘤质量、肺转移灶个数均显著降低(P0.01)。与空白组比较,化痰消瘤方及环磷酰胺能显著下调VEGF蛋白和VEGF mRNA的表达水平(P0.01),能显著上调nm-23蛋白和mRNA的表达水平(P0.01)。结论:化痰消瘤方具有抑制Lewis肺癌瘤体生长及转移的作用,其作用机制可能是通过降低VEGF的表达水平,减弱了肿瘤新生血管的生成,同时上调nm23 mRNA表达水平,抑制肿瘤生成与转移来实现的。

Effect of Huatan Xiaoliu Decoction on Expressions of nm-23 and VEGF in Lewis Tumor-bearing Mice

Objective:To observe the effect of Huatan Xiaoliu decoction (HXD) on tumor weight, lung metastases focuses, nm-23 and vascular endothelial growth factors(VEGF) in tumor tissues of Lewis tumor-bearing mice, and to explore its mechanism in restraining tumor growth and metastasis. Method:Totally 30 C57BL/6J male mice were randomly divided into the blank group, HXD group (50 g·kg^-1·d^-1), and Cyclophosphamide (CTX) group[0.06 g·kg^-1·(3,7 d)^-1], 10 mice in each group. Lewis lung cancer cells were inoculated subcutaneously into right axillary of C57BL/6 mice to establish Lewis tumor-bearing mice models. After 21 days, the mice were killed, and the tumors were stripped to calculate the rates of inhibition; their lung tissues were taken and after fixing, the number of lung metastases focuses was observed under the microscope to calculate the rate of anti-lung metastasis. The mRNA expressions of VEGF and nm-23 were detected by Real-time PCR, and the protein expressions of VEGF and nm-23 were detected by Western blot. Result:In comparison with blank group, the tumor weight and the number of lung metastasis focuses were significantly decreased in HXD group and CTX group (P<0.01); the mRNA and protein expressions of VEGF were decreased, and the mRNA and protein expressions of nm-23 were increased in HXD group and CTX group. Conclusion:Huatan Xiaoliu decoction could inhibit Lewis lung cancer growth and metastasis, and the underlying mechanism may be related to reducing the expression of VEGF, reducing the generation of tumor new vasculature, up-regulating the expression of nm-23, and inhibiting tumor generation and metastasis.