长链非编码RNA MALAT1在肝癌组织中的表达及其在肝癌细胞增殖与侵袭转移中的作用

目的:探讨长链非编码RNA MALAT1在肝癌组织中的表达及意义。方法:用qRT-PCR检测85例肝癌组织及其配对癌旁组织中MALAT1的表达,分析MALAT1的表达与肝癌患者临床病理因素的关系;在肝癌SMMC-7721细胞中分别过表达与敲除基因MALAT1后,通过CCK-8与Transwell实验检测肝癌细胞增殖与侵袭转移能力的改变。结果:MALAT1在肝癌组织中的表达明显高于癌旁正常组织(P0.01);MALAT1在肝癌组织中的表达与肿瘤大小、肝癌的分化程度、TNM分期、淋巴结转移明显有关(均P0.05)。肝癌SMMC-7721细胞过表达MALAT1后增殖、侵袭与转移能力明显增强,而敲低MALAT1后增殖、侵袭与转移能力明显减弱(均P0.05)。结论:MALAT1在肝癌组织中表达上调,MALAT1可促进肝癌细胞增殖、侵袭与转移,故可能与肝癌的发生发展密切相关。

Expression of long non-coding RNA MALAT1 in hepatocellular carcinoma tissue and its actions on proliferation and invasion/metastasis of hepatocellular carcinoma cells

Objective: To investigate the expression of long non-coding RNA MALAT1 in hepatocellular carcinoma (HCC) tissue and its significance.Methods: The MALAT1 expressions in 85 paired specimens of HCC and adjacent tissues were determined by qRT-PCR, and the relations of MALAT1 expression with clinicopathologic factors of the patients were analyzed. The changes in abilities of proliferation and invasion/metastasis in HCC SMMC-7721 cells after MALAT1 overexpression or knock-down were examined by CCK-8 and Transwell assay, respectively.Results: The MALAT1 expression in HCC tissue was significantly higher than that in cancer-adjacent normal tissue (P<0.01; the MALAT1 expression was significantly related to the tumor size, differentiation, TNM stage and lymph node metastasis of the patients (all P<0.05). In SMMC-7721 cells, the abilities of proliferation and invasion/metastasis were significantly enhanced after MALAT1 overexpression, and were significantly reduced after MALAT1 knock-down (all P<0.05).Conclusion: MALAT1 expression is up-regulated in HCC tissue, and it can promote proliferation and invasion/metastasis of HCC cells, so it may be closely associated with the occurrence and development of HCC.