| 补肾健脾活血方对去卵巢大鼠BMP2/Smad信号通路的影响 |
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| 引用本文： | 柴爽,王吉利,黄佳纯,黄宏兴,万雷,刘少津,汪悦东,胡仕畅.补肾健脾活血方对去卵巢大鼠BMP2/Smad信号通路的影响[J].中国实验方剂学杂志,2018,24(20):129-133. |
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| 作者姓名： | 柴爽 王吉利 黄佳纯 黄宏兴 万雷 刘少津 汪悦东 胡仕畅 |
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| 作者单位： | 广州中医药大学岭南医学研究中心中医骨伤科学实验室;广州中医药大学附属骨伤科医院 |
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| 基金项目： | 国家自然科学基金项目（81674004，81673786）；广东省科技计划项目（2016A020216024，2016ZC0096） |
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| 摘 要： | 目的:研究补肾健脾活血方对去卵巢大鼠骨形态发生蛋白2(BMP2)/Smad信号通路的影响。方法:72只6月龄雌性SD大鼠,随机分为假手术组(24只),手术组(48只),双侧卵巢切除法造模,术后3个月两组各随机选取12只检测骨密度验证造模成功。手术组剩余的36只大鼠随机分为双侧卵巢切除模型组(OVX),补肾健脾活血方组(BSJPHX,给药剂量2.979 g·kg~(-1)),阿仑膦酸钠维D3片(Ⅱ)组(ALN,给药剂量1.02 mg·kg~(-1)),假手术组,OVX组给与等体积生理盐水灌胃。12周后处死各组大鼠,双能X射线法检测大鼠全身骨密度,生物力学试验进行胫骨三点弯曲试验,实时荧光定量聚合酶链式反应(Real-time PCR)检测BMP2,Smad1,Runt相关转录因子2(Runx2),骨保护素(OPG)基因的表达,蛋白免疫印迹法(Western blot)检测BMP2,p-Smad1,Runx2,OPG蛋白的表达。结果:造模3个月后,与假手术组比较,OVX组大鼠骨密度明显降低,最大载荷及刚度均降低,BMP2,Smad1,Runx2,OPG基因和蛋白表达水平显著降低(P0.05);给药3个月后,与OVX组比较,BSJPHX组,ALN组骨密度均明显增高,最大载荷及刚度均增加,能显著提高BMP2,Smad1,Runx2,OPG基因和蛋白表达水平(P0.05)。结论:补肾健脾活血方既能通过调控BMP2/Smad通路的信号转导,又能上调OPG的表达,这可能是补肾健脾活血方防治绝经后骨质疏松症的机制之一。
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| 关 键 词： | 补肾健脾活血方 去卵巢大鼠 骨形态发生蛋白2(BMP2)/Smad信号通路 绝经后骨质疏松症 骨保护素 |
| 收稿时间： | 2018/4/18 0:00:00 |
Effect of Bushen Jianpi Huoxue Recipe on BMP2/Smad Signaling Pathway in Ovariectomized Rats |
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| CHAI Shuang,WANG Ji-li,HUANG Jia-chun,HUANG Hong-xing,WAN Lei,LIU Shao-jin,WANG Yue-dong and HU Shi-chang.Effect of Bushen Jianpi Huoxue Recipe on BMP2/Smad Signaling Pathway in Ovariectomized Rats[J].China Journal of Experimental Traditional Medical Formulae,2018,24(20):129-133. |
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| Authors: | CHAI Shuang WANG Ji-li HUANG Jia-chun HUANG Hong-xing WAN Lei LIU Shao-jin WANG Yue-dong and HU Shi-chang |
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| Institution: | Laboratory of Orthopaedics and Traumatology of Chinese Medicine of Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou 510006, China,Laboratory of Orthopaedics and Traumatology of Chinese Medicine of Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou 510006, China,Laboratory of Orthopaedics and Traumatology of Chinese Medicine of Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou 510006, China,The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China,The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China,Laboratory of Orthopaedics and Traumatology of Chinese Medicine of Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou 510006, China,Laboratory of Orthopaedics and Traumatology of Chinese Medicine of Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou 510006, China and Laboratory of Orthopaedics and Traumatology of Chinese Medicine of Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou 510006, China |
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| Abstract: | |
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| Keywords: | Bushen Jianpi Huoxue recipe ovariectomized rats bone morphogenetic protein 2(BMP2)/Smad signaling pathway postmenopausal osteoporosis (PMOP) osteoprotegerin (OPG) |
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