休克淋巴液对大鼠内皮细胞VEGF表达的影响

目的观察休克淋巴液对大鼠肺微血管内皮细胞(PMVEC)、肠系膜微淋巴管内皮细胞(MMLEC)、血管内皮生长因子(VEGF)表达的影响。方法无菌条件下复制大鼠重症失血性休克模型,引流休克时肠系膜淋巴液或收集门静脉血,同时收集正常淋巴液及门静脉血作为对照。以4%终浓度的处理因素与第3代PMVEC及MMLEC共同孵育6h,RT-PCR测定VEGF mRNA表达。结果不同处理因素与PMVEC及MMLEC孵育6h后,休克淋巴液组两种内皮细胞的VEGF mRNA表达均显著低于休克血浆组、正常淋巴液组、正常血浆组、胎牛血清(FBS)组、DMEM组;休克血浆组显著低于正常淋巴液组、正常血浆组、FBS组和DMEM组;其它组间无统计学差异。结论休克淋巴液可抑制内皮细胞的VEGF mRNA表达,且作用强于休克血浆。

Effect of Shock Lymph on VEGF Expression of Endothelial Cells

Objective To observe the effects of shock lymph on vascular endothelial growth factor(VEGF) expression of pulmonary micro-vascular endothelial cells(PMVEC) and mesentery micro-lymphatic endothelial cells(MMLEC). Methods The model of serious hemorrhagic shock was established in the axenic condition, mesentery lymph and portal vein blood were collected during shock. As control, mesentery lymph and portal vein blood were collected in normal condition. The primary PMVEC and MMLEC of passages 3 were treated by different factors at the final concentration of 4% for 6h, respectively. The expression of VEGF mRNA was measured by RT-PCR method. Results After PMVEC and MMLEC were treated by different factors for 6h, the VEGF mRNA expressions of PMVEC and MMLEC in shock lymph group were obviously lower than those of shock plasma group, normal lymph group, normal plasma group, fetal bovine serum(FBS) group and DMEM group, and the VEGF mRNA expressions of PMVEC and MMLEC in shock plasma group were obviously lower than those of normal lymph group, normal plasma group, FBS group and DMEM group. There was no statistical difference among the other groups. Conclusion The results demonstrated that the shock lymph could reduce the VEGF mRNA expressions of PMVEC and MMLEC, and the reduction was more effective than that of shock plasma.