新生儿脐血和母血中维生素E值的观察——分光光度法测定

本文用微量分光光度法对57例新生儿脐血和30例产妇血维生素E浓度进行测定,并以30例正常成人为对照。本测定方法是可靠和灵敏的。平均回收率为93.8％(92.2～98.0％),变异系数(CV)在高浓度和“低浓度标本重复测定时分别为2.5％和7.0％。脐血中E值平均为2.8±0.9gμ/ml,约为成人值的1/3。分娩时母血维生素E值明显高于正常未孕妇女(P<0.001)为12±2.5μg/ml。母血维生素E值和其配对新生儿脐血维生素E值相关(r=0.44887,P<0.01)。

SPECTROPHOTOMETRIC DETERMINATION OF VITAMIN E IN CORD AND MATERNAL BLOOD

Serum vitamin E was determined using bathopllenanthroline by microspe-ctrophotometric method. The average recovery rate was 93.8 (92.2-98.0) per cent and the coefficients of variation were 2.5 and 7 per cent in high and low levels respectively. This method seems to be quite reliable and sensitive. Of the total 117 serum samples, 30 pairs matched blood for mother and cord, 27 cord blood, and 30 normal adults as control were studied. The mean level of vitamin E in the cord blood was 2.8 ug/ml (±0.9SD), which was about one third of that in the adult. The level of vitamin E in post-partum mother was 12 μg/ml (±2.5 SD), which was significantly higher than that of nonpregnant women (p<0.001). The vitamin E concentration was found correlating well with that of the cord blood (r = 0.4887, p<0.01).