| 近红外光谱用于卷柏属药用植物种下类型鉴别及种间关系探讨 |
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| 引用本文： | 万定荣,江燕,陈科力.近红外光谱用于卷柏属药用植物种下类型鉴别及种间关系探讨[J].中华中医药杂志,2008,23(11):967-970. |
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| 作者姓名： | 万定荣 江燕 陈科力 |
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| 作者单位： | [1]中南民族大学生命科学学院,武汉430074 [2]湖北省药品检验所,武汉430064 [3]湖北中医学院药学院,武汉430065 |
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| 摘 要： | 目的:研究解决卷柏属植物药分类鉴定巾的难点问题,探讨该属药用种间亲缘关系.方法:应用傅立叶变换近红外光谱仪、固体光纤探头、InGaAs检测器,扫描范围4000-900cm-1,每份样品扫描32次,并重复测定5次求平均值.平均光谱经处理后转换成一阶导数光谱,再用OPUS/INDENT软件进行聚类分析.并获取2类薄叶卷柏样品的二阶导数光谱.结果:根据光谱图及聚类图,薄叶卷柏和异穗卷柏各2个类型均各为同种,且薄叶卷柏变异类型应视为变种.垫状卷柏与卷柏亲缘关系密切,同作卷柏药用合理.江南卷柏与薄叶卷柏关系较近,民间同作一药有合理性.结论:近红外光谱可简单快速地用于解决药用植物种下分类鉴定的一些疑点问题,可推断某些种间亲缘关系.
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| 关 键 词： | 近红外光谱 卷柏属 药用植物 分类鉴定 亲缘关系 |
Discussion on identification and phylogenetic relationships of medicinal plants in Selaginellaceae by NIRS |
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| WAN Ding-rong,JIANG Yan,CHEN Ke-li.Discussion on identification and phylogenetic relationships of medicinal plants in Selaginellaceae by NIRS[J].China Journal of Traditional Chinese Medicine and Pharmacy,2008,23(11):967-970. |
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| Authors: | WAN Ding-rong JIANG Yan CHEN Ke-li |
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| Institution: | WAN Ding-rong1,JIANG Yan2,CHEN Ke-li3 ( 1 College of Life Science,South-Central University for Nationalities,Wuhan 430074,China,2 Hubei Institute for Drug Control,Wuhan 430064,3Department of Pharmacy,Hubei College of TCM,Wuhan 430065,China) |
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| Abstract: | Objective:to study and solve some difficult problems in identification of medicinal plants in Selaginellaceae, and research the phylogenetic relationships among them.Methods:All the spectra were collected by using a Fourier transform near-infrared spectrometer equipped with a fiber-optic probe and a InGaAs detector for reflectance measurements.All the samples were scanned from 9 000 cm-1 to 4 000 cm-1,and each sample spectrum was obtained as an automatic mean of 32 times.The samples were measured 5 times.Th... |
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| Keywords: | Near-infrared spectrometer Selaginellaceae Medicinal plant Classification and identification Genetic relationship |
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