HPLC法同时测定血清中苯巴比妥、苯妥英和卡马西平的浓度 |
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引用本文: | 陈秋虹,何林,曾明辉,汪震. HPLC法同时测定血清中苯巴比妥、苯妥英和卡马西平的浓度[J]. 西北药学杂志, 2001, 16(1): 3-4 |
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作者姓名: | 陈秋虹 何林 曾明辉 汪震 |
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作者单位: | 1. 四川省人民医院药剂科 2. 四川省人民医院药剂科;华西医科大学临床药学专业99届实习生 |
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摘 要: | 采用 RP-HPLC法,以甲醇:乙腈:水(40: 8: 52)为流动相,检测波长254nm,采用外标法同时测定血清中苯巴比妥、苯妥英和卡巴西平浓度。结果苯巴比妥、苯妥英和卡马西平浓度分别在3.97~79.42μg/ml,2.01~80.24μg/ml, 1. 02~40.9μg/ml浓度范围内呈线性关系。
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关 键 词: | 反相高效液相色谱法 苯巴比妥 苯妥英 卡马西平 |
修稿时间: | 2000-07-10 |
Simultaneous determinationof phenobarbital, phenytoin and carbamazepine in serum by RP-HPLC |
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Abstract: | The concentrations of phenobarbital, phenytoin and carbamazepine in serum were determined simultaneously by RP-HPLC with external standard method. The mobile phase consisted of the mixture of methanol, acetonitrile and water(40∶8∶52). The detector set at 254nm. The results showed the linear concentrations of phenobarbital, phenytoin and carbamazepine were in the range of 7.94~79.42μg/ml, 8.02~80.24μg/ml, 2.05~40.9μg/ml respectively. |
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Keywords: | RP-HPLC phenobartital phenytoin carbamazepine |
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