自然杀伤T细胞与子宫内膜异位症分期的关系

摘要：目的探讨自然杀伤T细胞(NKT)在子宫内膜异位症(EMT)发病机制的作用及其与分期的关系。方法Ⅰ~Ⅳ期EMT患者
各15例，采用流式细胞术检测其外周血及腹腔积液内NKT含量，用ELISA法检查细胞因子IFN-γ及IL-4的含量，并选取同龄段
的20例健康志愿者作为对照。结果与对照组比较，Ⅰ~Ⅳ期EMT患者外周血NKT、IFN-γ及IL-4均明显降低，且外周血及腹腔
积液内以上三者的含量均呈现Ⅰ>Ⅱ>Ⅲ>Ⅳ的关系，两组间的含量存在统计学差异（P<0.05）。Spearman 相关性分析显示，
NKT、IFN-γ及IL-4当中任意一个因素均与Ⅰ、Ⅱ、Ⅲ及Ⅳ的任意一期呈显著负相关性（r均大于0.06，P均小于0.05）。结论NKT
及其细胞因子IFN-γ及IL-4在EMT发病机制当中具有重要作用，并且与EMT的分期密切相关，可能是该病的一组保护性因素。

Association of natural killer T cells with staging of endometriosis

Objective To investigate the role of natural killer T cells(NKT) in the pathogenesis and staging of endometriosis(EMT).Methods Sixty EMT cases of stages I to IV were enrolled as the observation group,and 20 healthy volunteers served as the control group.NKT percentages in the peripheral blood and peritoneal effusions were detected by flow cytometry,and the levels of interferon-γ(IFN-γ) and interleukin-4(IL-4) were examined using ELISA.Results Compared with the control group,EMT group showed significantly lowered NKT percentages and IFN-γ and IL-4 levels in both the peripheral blood and peritoneal effusions(P<0.05).In EMT patients,NKT percentages,IFN-γ and IL-4 levels all increased significantly with the stage of EMT in the order of Ⅰ>Ⅱ>Ⅲ>Ⅳ(P<0.05).Spearman correlation analysis showed that NKT,IFN-γ and IL-4 were all inversely correlated with the stage of EMT(r>0.06,P<0.05).Conclusion As a protective factor against EMT,NKT,together with its cytokines IFN-γ and IL-4,play an important role in EMT and is closely correlated with EMT staging.