| 基于UPLC-TOF/MS分析人参附子配伍减毒的物质基础 |
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| 作者姓名： | Ma ZC Zhou SS Liang QD Huo C Wang YG Tan HL Xiao CR Gao Y |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所; |
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| 基金项目： | 国家重点基础研究发展计划资助项目(2012CB518402,2011CB505304); 北京市自然科学基金资助项目(7112110) |
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| 摘 要： | 利用超高效液相色谱-飞行时间质谱联用技术(UPLC-TOF/MS)分析人参附子药对配伍减毒的物质基础,从化学成分层次阐释其配伍减毒机制。基于UPLC-TOF/MS建立人参附子药对配伍后生物碱类成分的化学指纹图谱,通过主成分分析法和正交偏最小二乘判别法分析药对配伍在合煎过程中的生物碱类成分的含量变化,找出差异变化显著的化学成分。结果表明,正离子模式时人参附子药对合煎液中次乌头碱、去氧乌头碱的含量明显降低,而苯甲酰中乌头原碱、苯甲酰次乌头原碱和去乙酸中乌头原碱等含量升高。人参附子药对配伍应用时双酯型二萜生物碱的含量明显降低,而单酯型二萜生物碱的含量明显升高,这可能是人参附子药对配伍减毒作用的物质基础。
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| 关 键 词： | 人参 附子 配伍 |
UPLC-TOF/MS based chemical profiling approach to evaluate toxicity-attenuated chemical composition in combination of ginseng and radix aconiti praeparata |
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| Ma ZC,Zhou SS,Liang QD,Huo C,Wang YG,Tan HL,Xiao CR,Gao Y.UPLC-TOF/MS based chemical profiling approach to evaluate toxicity-attenuated chemical composition in combination of ginseng and radix aconiti praeparata[J].Acta Pharmaceutica Sinica,2011,46(12):1488-1492. |
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| Authors: | Ma Zeng-Chun Zhou Si-Si Liang Qian-De Huo Chao Wang Yu-Guang Tan Hong-Ling Xiao Cheng-Rong Gao Yue |
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| Institution: | MA Zeng-chun,ZHOU Si-si,LIANG Qian-de,HUO Chao,WANG Yu-guang,TAN Hong-ling,XIAO Cheng-rong,GAO Yue(Beijing Institute of Radiation Medicine,Beijing 100850,China) |
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| Abstract: | In the present study,an ultra performance liquid chromatography coupled with time-of-fight mass spectrometry(UPLC-TOF/MS) based chemical profiling approach was used to evaluate chemical constitution between co-decoction and mixed decoction of ginseng and Radix Aconiti Praeparata.Two different kinds of decoctions,namely co-decoction of ginseng and Radix Aconiti Praeparata: water extract of mixed two herbs,and mixed decoction of ginseng and Radix Aconiti Praeparata: mixed water extract of each individual herb... |
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| Keywords: | ginseng Radix Aconiti Praeparata compatibility |
| 本文献已被 CNKI PubMed 等数据库收录！ |
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