| siRNA非病毒递送载体的研究现状 |
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| 引用本文： | Yang FF,Huang W,Li YF,Gao ZG. siRNA非病毒递送载体的研究现状[J]. 药学学报, 2011, 46(12): 1436-1443 |
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| 作者姓名： | Yang FF Huang W Li YF Gao ZG |
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| 作者单位： | 中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室; |
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| 基金项目： | 国家自然科学基金资助项目(30873168) |
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| 摘 要： | RNA干扰(RNA interference,RNAi)是近年发展起来的一种新技术。RNAi是指通过外源性或内源性的双链RNA在体内诱导靶基因mRNA产生特异性降解,进而引起不同水平的基因沉默。RNAi已经用于肿瘤、病毒感染、乙型肝炎等多种疾病的治疗。小干扰RNA(siRNA)是RNAi的效应分子,可在体内诱导RNAi效应。但是裸siRNA在体内容易被核酶(RNase)降解,且半衰期短,转染效率低。因此,siRNA需要借助递送载体进入细胞发挥治疗作用。病毒载体在基因治疗中有潜在的免疫原性、致突变等副作用。所以,非病毒载体成为当前的研究热点。本文对siRNA非病毒递送载体的研究现状进行了综述。
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| 关 键 词： | RNAi siRNA 非病毒载体 |
Current status of non-viral vectors for siRNA delivery |
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| Yang Fei-Fei,Huang Wei,Li Yun-Fei,Gao Zhong-Gao. Current status of non-viral vectors for siRNA delivery[J]. Acta pharmaceutica Sinica, 2011, 46(12): 1436-1443 |
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| Authors: | Yang Fei-Fei Huang Wei Li Yun-Fei Gao Zhong-Gao |
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| Affiliation: | YANG Fei-fei,HUANG Wei,LI Yun-fei,GAO Zhong-gao(State Key Laboratory of Bioactive Substances and Functions of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100050,China) |
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| Abstract: | RNA interference(RNAi) is a newly developed technology.It is the different levels of gene silencing induced by specific degradation of targeted genes in vivo,and both exogenous and endogenous double-stranded RNAs could induce the specific degradation.RNAi has been applied in tumor therapy,viral infection,hepatitis B and many other diseases.siRNA is the effector molecule which induces the RNAi in vivo.But naked siRNA is easily degradated by RNases in vivo,and the half-life is short.Meanwhile,the transfection... |
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| Keywords: | RNAi siRNA non-viral vector |
| 本文献已被 CNKI PubMed 等数据库收录！ |
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