| 液相色谱-串联质谱法测定头发中11种阿片类生物碱 |
| |
| 引用本文： | Sun YY,Xiang P,Shen M. 液相色谱-串联质谱法测定头发中11种阿片类生物碱[J]. 药学学报, 2011, 46(12): 1501-1506 |
| |
| 作者姓名： | Sun YY Xiang P Shen M |
| |
| 作者单位： | 苏州大学基础医学与生物科学学院法医学系;司法部司法鉴定科学技术研究所上海市法医学重点实验室; |
| |
| 基金项目： | 国家自然科学基金资助项目(20975070); 国家科研院所公益项目(GY0903) |
| |
| 摘 要： | 建立头发中海洛因、吗啡、单乙酰吗啡等11种阿片类生物碱的液相色谱-串联质谱测定方法,并考察海洛因滥用者头发中阿片类组分的存在情况。头发经冷冻研磨后加入硼酸缓冲液超声30 min,用氯仿-异丙醇(9∶1)提取。用Allure PFP丙基柱,以乙腈-乙酸铵(0.1%甲酸)梯度洗脱分离,采用二级质谱多反应监测模式(MRM)检测11种阿片类生物碱。头发中海洛因、吗啡、单乙酰吗啡等11种阿片类生物碱在对应质量浓度范围内线性良好(r>0.996 0);检测限(LOD)均小于0.05 ng.mg-1;回收率范围为47.2%～110%;日内精密度和日间精密度均小于14%。21例海洛因滥用者头发中均检出了海洛因、单乙酰吗啡、吗啡、可待因、乙酰可待因、氢可酮等主要组分。所建方法灵敏度高、选择性好,适用于同时分析头发中海洛因等11种阿片类生物碱组分,可有效鉴别海洛因滥用与阿片类药物或食品的摄取。
|
| 关 键 词： | 头发 阿片类生物碱 LC-MS/MS |
Simultaneous determination of 11 opiates in hair by liquid chromatography-tandem mass spectrometry |
| |
| Sun Ying-Ying,Xiang Ping,Shen Min. Simultaneous determination of 11 opiates in hair by liquid chromatography-tandem mass spectrometry[J]. Acta pharmaceutica Sinica, 2011, 46(12): 1501-1506 |
| |
| Authors: | Sun Ying-Ying Xiang Ping Shen Min |
| |
| Affiliation: | SUN Ying-ying1,2,XIANG Ping2,SHEN Min2(1.Department of Forensic Medicine,Institute of Preclinical Medicine and Biological Science,Soochow University,Suzhou 215123,China,2.Shanghai Key Laboratory of Forensic Science,Institute of Forensic Science,Ministry of Justice,Shanghai 200063,China) |
| |
| Abstract: | The paper reports the establishment of a liquid chromatography-tandem mass spectrometry(LC-MS/MS) for simultaneous analysis of 11 opiates in hair samples,and the study of presence of opiates in the hair of active heroin addicts.About 20 mg of decontaminated and pulverized hair sample was hydrolyzed with buffer solution for 30 min,in the presence of morphine-d3 and acetylmorphine-d6 used as internal standards,and then extracted with the mixture of dichlormethane and isopropanol,separated by the Allure PFP pr... |
| |
| Keywords: | hair opiate LC-MS/MS |
| 本文献已被 CNKI PubMed 等数据库收录！ |
|
