扇贝裙边糖胺聚糖对过氧化氢损伤的内皮细胞释放血管活性物质的影响

目的:观察扇贝裙边糖胺聚糖(SS-GAG)对过氧化氢(H2O2)损伤的血管内皮细胞产生血管活性物质的影响。方法:用体外培养的人脐静脉内皮细胞株ECV304建立H2O2诱导的内皮细胞损伤模型组、不同浓度的SS-GAG组以及正常对照组,用放射免疫法测定培养液中6-酮-前列腺素F1a(6-keto-PGF1a)、血栓素B2(TXB2)、内皮素(ET)的含量;用硝酸还原酶法测定一氧化氮(NO)的含量。结果:与模型组比较,不同浓度的SS-GAG组均可使血管舒张因子6-keto-PGF1a、NO含量明显增加;缩血管因子TXB2、ET含量明显减少(P<0.05,P<0.01)。结论:SS-GAG可能通过增加舒血管因子、降低缩血管因子的分泌来减轻H2O2对内皮细胞的氧化损伤。

Effects of Glycosaminoglycan from Scallop Skirt on the Releasing of Vasoactive Substance from Endothelial Cells Injured by Hydrogen Peroxide

OBJECTIVE： To observation the effects of Glycosaminoglycan from Scallop Skirt （SS-GAG） on the production of vasoactive substance in the endothelial cells injured by hydrogen peroxide（H2O2）. METHODS： In H2O2-induced endothelial cell oxidative damage model group which was established with human umbilical vein endothelial cell （HUVEC, ECV304） cultured in vitro, SS - GAG groups （at different concentrations） and the normal group, levels of 6-keto-PGF1a, Thromboxane-B2 and Endothelin （ET） in the culture fluid were detected by radioimmunity assay and the content of nitric oxide was determined by nitrate reductase method. RESULTS： As compared with model group, SS-GAG （at different concentrations） significantly enhanced the levels of 6-keto-PGF1a and NO but significantly decreased levels of TXB2 and ET （P 〈 0.05, P〈 0.01） . CONCLUSION： SS-GAG can lessen the oxidative damage of endothelial cells induced by H2O2 by increasing levels of 6-keto-PGF1a and NO and decreasing expression of TXB2 and ET.