miR-23c靶向调控MTDH抑制胶质瘤U87细胞增殖迁移与侵袭

目的 探讨miR-23c对胶质瘤细胞U87增殖、迁移和侵袭的影响及初步机制。方法 将miR-23c mimics转染于U87细胞中，用miR-无关序列转染于U87细胞做为Control组，采用MTT法、细胞划痕、Transwell侵袭实验观察miR-23c对U87细胞增殖、迁移和侵袭的影响；采用生物信息学软件分析miR-23c潜在的靶基因；采用Western blot、RT-PCR和萤光素酶报告基因检测miR-23c对靶基因的调控作用；在转染miR-23c mimics的基础上同时转染MTDH质粒，通过MTT法、细胞划痕、transwell侵袭实验观察转染MTDH对miR-23c抑制U87细胞增殖、迁移和侵袭的影响。结果 MTT实验显示，miR-23c组U87细胞的OD值为（0.668±0.032），明显低于Control组的（1.031±0.060）（P<0.01）；细胞划痕实验显示，miR-23c组细胞划痕愈合率（0.35±0.02）明显低于对照组的（0.59±0.03）（P<0.01）；Transwell侵袭实验显示，miR-23c组U87细胞穿过基质胶的细胞数（153.2±8.30）明显低于与对照组的（348.4±12.12）（P<0.01）；Western blot、RT-PCR和萤光素酶报告基因检测表明MTDH是miR-23c直接调控的靶基因；MTT实验显示，miR-23c+MTDH组U87细胞的OD值为（1.025±0.059），明显高于miR-23c组的（0.672±0.024）（P<0.01）；细胞划痕实验显示，miR-23c+MTDH组细胞划痕愈合率为（0.45±0.04），明显高于miR-23c组的（0.31±0.03）（P<0.05）；Transwell侵袭实验显示，miR-23c+MTDH组U87细胞穿过基质胶的细胞数（260.9±10.23），明显高于miR-23c组的（148.4±9.4）（P<0.01）。结论 上调miR-23c能明显抑制胶质瘤细胞的增殖、迁移和侵袭能力，其机制可能与其下调MTDH表达有关。

miR-23c inhibits the proliferation,migration,and invasion of glioma U87 cells via targeted regulation of MTDH

Objective To investigate the effect of miR-23c on the proliferation, migration, and invasion of glioma U87 cells and preliminary mechanisms.Methods U87 cells were transfected with miR-23c mimics, and the U87 cells transfected with miR-negative control were established as control group. MTT assay, wound healing assay, and Transwell invasion experiment were used to observe the effect of miR-23c on the proliferation, migration, and invasion of U87 cells. Bioinformatics software was used to analyze the potential target genes of miR-23c, and Western blot, RT-PCR, and luciferase reporter gene assay were used to observe the regulatory effect of miR-23c on target genes. After U87 cells were co-transfected with miR-23c mimics and MTDH, MTT assay, wound healing assay, and Transwell invasion experiment were used to analyze the effect of MTDH transfection on the proliferation, migration, and invasion of U87 cells inhibited by miR-23c.Results The MTT assay showed that the miR-23c group had a significantly lower OD value of U87 cells than the control group (0.668±0.032 vs 1.031±0.060,P<0.01); the wound healing assay showed that the miR-23c group had a significantly lower wound healing rate than the control group (0.35±0.02 vs 0.59±0.03,P<0.01); the Transwell invasion experiment showed that the miR-23c group had a significantly lower number of U87 cells passing through the matrigel than the control group (153.2±8.30 vs 348.4±12.12,P<0.01). Western blot, RT-PCR, and luciferase reporter gene assay showed that MTDH was a target gene directly regulated by miR-23c. The MTT assay showed that the miR-23c+MTDH group had a significantly higher OD value of U87 cells than the miR-23c group (1.025±0.059 vs 0.672±0.024,P<0.01); the wound healing assay showed that the miR-23c+MTDH group had a significantly higher wound healing rate of U87 cells than the miR-23c group (0.45±0.04 vs 0.31±0.03,P<0.05); the Transwell invasion experiment showed that the miR-23c+MTDH group had a significantly higher number of U87 cells passing through the matrigel than the miR-23c group (260.9±10.23 vs 148.4±9.4,P<0.01).Conclusions Upregulation of miR-23c can significantly inhibit the proliferation, migration, and invasion of glioma cells, possibly by downregulating the expression of MTDH.