| 高效液相色谱法测定银翘解毒颗粒中绿原酸含量 |
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| 引用本文： | 冯兵,钱妍.高效液相色谱法测定银翘解毒颗粒中绿原酸含量[J].中国药业,2011,20(11):19-20. |
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| 作者姓名： | 冯兵 钱妍 |
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| 作者单位： | 1. 重庆市急救医疗中心药剂科,重庆,400014
2. 重庆医科大学第二附属医院药剂科,重庆,400010 |
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| 摘 要： | 目的建立测定银翘解毒颗粒绿原酸含量的高效液相色谱法。方法色谱柱为Kromasil KR100-5C柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.4%磷酸溶液(11∶89),柱温25℃,检测波长327 nm。结果绿原酸的检测进样量在0.064 5～0.516 0μg范围内与峰面积呈良好的线性关系(r=1.000 0),平均加样回收率为100.87%,RSD=1.98%(n=7)。结论所用方法简单、快捷准确,可作为银翘解毒颗粒的质量控制方法。
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| 关 键 词： | 高效液相色谱法 银翘解毒颗粒 绿原酸 含量测定 |
Content Determination of Chlorogenic Acid in Yinqiaojiedu Particles by HPLC |
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| Feng Bing,Qian Yan.Content Determination of Chlorogenic Acid in Yinqiaojiedu Particles by HPLC[J].China Pharmaceuticals,2011,20(11):19-20. |
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| Authors: | Feng Bing Qian Yan |
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| Institution: | Feng Bing1,Qian Yan2(1.Department of Pharmacy,Chongqing Emergency Medical Center,Chongqing,China 400014,2.Department of Pharmacy,Second Affiliated Hospital of Chongqing Medical University,China 400010) |
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| Abstract: | Objective To determine the content of chlorogenic acid in Yinqiaojiedu Particles by HPLC.Methods The analytical column was Kromasil KR100-5C（250 mm×4.6 mm,5 μm）,mobile phase was acetonitrile-0.4%phosphoric acid（11 ∶89）,column temperature was 25 ℃,the detection wavelength was 327 nm.Results The chlorogenic acid concentration was linear within the range of 0.064 5-0.516 0 μg（r=1.000 0）,the average recovery rate was 100.87%,RSD=1.98%（n=7）.Conclusion This method is simple,quick and accurate,and can be used for quality control of Yinqiaojiedu Particles. |
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| Keywords: | HPLC Yinqiaojiedu Particles chlorogenic acid content determination |
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