| 响应面法优化白囊耙齿菌中腺苷的超声法提取工艺 |
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| 引用本文： | 张娜,郭姣,季文彬,刘天洋,高朝辉.响应面法优化白囊耙齿菌中腺苷的超声法提取工艺[J].中国医药工业杂志,2011,42(2). |
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| 作者姓名： | 张娜 郭姣 季文彬 刘天洋 高朝辉 |
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| 作者单位： | 吉林大学生命科学学院,吉林长春,130012 |
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| 摘 要： | 应用响应面法优化白囊耙齿菌中腺苷的超声法提取工艺.在单因素试验的基础上,采用3因素3水平的Box-Behnken中心组合设计和响应面分析法,考察超志声提取时间、超声功率和液料比对腺苷得率的影响,并优化腺苷超声法提取条件.白囊耙齿菌中腺苷的最佳超声提取条件为:提取时间378s、功率414 W、液料比37:l(v/w),理论提取率为0.88%,实际提取率为0.89%,相对误差为1.14%.
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| 关 键 词： | 腺苷 白囊耙齿菌 响应面法 超声提取 |
Optimal Conditions of Ultrasonic-assisted Extraction of Adenosine from Irpex lacteus Using Response Surface Method |
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| Authors: | ZHANG Na GUO Jiao JI Wenbin LIU Tianyang GAO Chaohui |
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| Institution: | ZHANG Na,GUO Jiao,JI Wenbin,LIU Tianyang,GAO Chaohui~* (College of Life Science,Jilin University,Changchun 130012) |
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| Abstract: | A response surface analysis method was applied to optimize the ultrasonic conditions for extraction of adenosine in Irpex lacteuss mycelia.Based on the single factor experiments,a three-factor-three-level experiment was designed by Box-Behnken central composite design method.The influences of extraction time,ultrasonic power and solvent/solid ratio on the yield of adenosine were studied.The response surface method was employed to analyze the results of experiments.The results indicated that the optimum ultr... |
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| Keywords: | adenosine Irpex lacteus response surface method ultrasonic-assisted extraction |
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