| 埃坡霉素高产菌株的选育及发酵培养基的优化 |
| |
| 引用本文： | 王向阳,陈少欣.埃坡霉素高产菌株的选育及发酵培养基的优化[J].中国医药工业杂志,2011,42(4). |
| |
| 作者姓名： | 王向阳 陈少欣 |
| |
| 作者单位： | 上海医药工业研究院,上海,200040 |
| |
| 基金项目： | 国家"重大新药创制"创新药物研究与开发技术休系建设，国家"重人新药创制"科技重大专项资助 |
| |
| 摘 要： | 以埃坡霉素产生菌粘细菌纤维堆囊菌为出发菌株,经亚硝基胍诱变处理后,在含有前体丙酸盐的平板中筛选抗性突变株,得到一株高产突变株N-19,埃坡霉素产量为18.5 mg/L,比出发菌株提高了69.7.通过优化发酵培养基的碳、氮源组成,埃坡霉素产量达36.1 mg/L,此时埃坡霉素B和埃坡霉素A的比值为0.4:在摇瓶发酵过程中补加前体丙酸盐,埃坡霉素B和埃坡霉素A的比值增至2.1.
|
| 关 键 词： | 埃坡霉素 粘细菌纤维堆囊菌 菌种选育 发酵 |
Breeding of High Productivity Strains of Epothilones and Optimization of Fermentation Medium |
| |
| Authors: | WANG Xiangyang CHEN Shaoxin |
| |
| Institution: | WANG Xiangyang,CHEN Shaoxin(Shanghai Institute of Pharmaceutical Industry,Shanghai 200040) |
| |
| Abstract: | The epothilones-producing strain Sorangium cellulosum was mutated with NTG and screened in the plate containing propionate,the precusor of epothilones.A mutant N-19 with the propionate resistant was obtained,whose production of epothilones was 18.5 mg/L,an increase of 69.7% compared with that of the parent strain.Through further optimization of the fermentation medium,the yield of epothilones was 36.1 mg/L and the ratio of epothilone B to epothilone A was 0.4.After bacth-feeding of propionate during the fer... |
| |
| Keywords: | epothilones Sorangium cellulosum strain breeding fermentation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
