| 高效液相色谱法测定五苓片中肉桂酸含量 |
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| 引用本文： | 袁祥慧,杨惠莲.高效液相色谱法测定五苓片中肉桂酸含量[J].中国药业,2011,20(9):18-19. |
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| 作者姓名： | 袁祥慧 杨惠莲 |
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| 作者单位： | 重庆市药品检验所,重庆,401121 |
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| 摘 要： | 目的 建立测定五苓片中肉桂酸含量的高效液相色谱法.方法 色谱柱采用Diamonsil C18柱(250mm× 4.6 mm,5μm),以乙腈-0.1%磷酸溶液(30:70)为流动相,流速1.0mL/min,检测波长285nm.结果 肉桂酸进样量在0.009 608～0.288 24μg范围内与峰面积线性关系良好,r=0.9999(n=6);平均回收率为96.39%,RSD为0.44%(n=6).结论 高效液相色谱法专属性强、操作简便、结果 准确、重现性好,可用于测定五苓片中肉桂酸的含量.
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| 关 键 词： | 五苓片 肉桂酸 高效液相色谱法 含量测定 |
Determination of Cinnamic Acid in Wuling Tablets by HPLC |
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| Yuan Xianghui,Yang Huilian.Determination of Cinnamic Acid in Wuling Tablets by HPLC[J].China Pharmaceuticals,2011,20(9):18-19. |
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| Authors: | Yuan Xianghui Yang Huilian |
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| Institution: | Yuan Xianghui,Yang Huilian(Chongqing Institute for Drug Control,Chongqing,China 401121) |
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| Abstract: | Objective To establish an RP-HPLC method for the determination of cinnamic acid in Wuling Tablets.Methods The RP-HPLC method was adopted and Diamonsil C18 column（250 mm×4.6 mm,5 μm）was used.The mobile phase was acetonitrile-0.1% phosphric acid（30：70） with the flow rate of 1.0 mL/min.The UV detection wavelength was set at 285 nm.Results The calibration curve of cinnamic acid was linear in the range of 0.009 608-0.288 24 μg.The average recover rate was 96.39%,RSD=0.44%（n=6）.Conclusion The method is specific,simple,accurate and has good repeatability.It can be used to determinate the cinnamic acid in Wuling Tablets. |
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| Keywords: | Wuling Tablets cinnamic acid HPLC content determination |
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