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| 芘荧光探针法结合曲线拟合研究胆盐-磷脂混合纳米胶束的聚集行为 | |
| 引用本文： | 陆国庆,郑江,郑新川,鲁永玲,何凤慈.芘荧光探针法结合曲线拟合研究胆盐-磷脂混合纳米胶束的聚集行为[J].中国药业,2011,20(10):21-23. |
| 作者姓名： | 陆国庆 郑江 郑新川 鲁永玲 何凤慈 |
| 作者单位： | 1. 中国人民解放军第三军医大学科研部,重庆,400038;中国人民解放军成都军区总医院临床药学科,四川,成都,610083 2. 中国人民解放军第三军医大学西南医院中心实验室,重庆,400038 3. 中国人民解放军第三军医大学科研部,重庆,400038 |
| 摘 要： | 目的研究不同磷脂比例的胆盐-磷脂混合纳米胶束的聚集行为。方法以芘为荧光探针,测定不同磷脂比例的胆盐-磷脂混合纳米胶束增溶芘后的荧光光谱,以I1/I3值(I1=372 nm,I3=383 nm)为纵坐标、胶束浓度对数值logC为横坐标,利用Origin8.0软件进行曲线拟合,以S型曲线的突变中点切线和平行切线的交点定义临界胶束浓度。结果磷脂/胆盐比例(W/W)为2∶1,1∶1,1∶2的混合胶束临界胶束浓度分别为0.034,0.048,0.055 g/L。结论提高混合胶束中磷脂比例,可以减小胶束的临界胶束浓度,有利于胶束发挥更大的增溶作用。 |
| 关 键 词： | 芘 荧光探针 胆盐 磷脂 临界胶束浓度 |
The Study of Aggregation Behavior of Bile Salt-phosphatidylcholine Mixed Nano-micelle by Pyrene Fluorescence Probe Spectrometry Methods and Curve Fitting |
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| Lu Guoqing,Zheng Jiang,Zheng Xinchuan,Lu Yonglin,He Fengci.The Study of Aggregation Behavior of Bile Salt-phosphatidylcholine Mixed Nano-micelle by Pyrene Fluorescence Probe Spectrometry Methods and Curve Fitting[J].China Pharmaceuticals,2011,20(10):21-23. | |
| Authors: | Lu Guoqing Zheng Jiang Zheng Xinchuan Lu Yonglin He Fengci |
| Institution: | Lu Guoqing1,2,Zheng Jiang3,Zheng Xinchuan3,Lu Yonglin3,He Fengci1(1.Division of Scientific Research Affairs,Third Military Medical University,Chongqing 400038,China,2.Department of Clinical pharmacy,Chengdu Miilitary General Hospital,Chengdu 610083,3.Medical Research Center,Southwestern Hospital,China) |
| Abstract: | Objective The aim of current study was to study the aggregation behavior of bile salt-phosphatidylcholine mixed nano-micelle prepared by different phospholipid fraction.Methods The fluorescence spectra of different phospholipid fraction of bile salt-phosphatidylcholine mixed nano-micelle were measured,in which pyrene was solubilized and used as fluorescence probe.I1/I3(I1=372 nm,I3=383 nm),according to the curves of fluorescence spectra,was taken as Y-axis and logC as X-axis simultaneously.The plot of pyren... |
| Keywords: | pyrene fluorescence probe bile salt phospholipid critical micelle concentration |
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