| 高效液相色谱法测定苦参片中苦参碱含量 |
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| 引用本文： | 周世玉,周晓英,唐婧坤.高效液相色谱法测定苦参片中苦参碱含量[J].中国药业,2011,20(7):27-28. |
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| 作者姓名： | 周世玉 周晓英 唐婧坤 |
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| 作者单位： | 1. 四川省成都市药品检验所,四川,成都,610045
2. 泸州医学院,四川,泸州,646000 |
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| 摘 要： | 目的建立测定苦参片中苦参碱含量的高效液相色谱法。方法色谱柱采用AlltechAlltimaAmino柱(250 mm×4.6 mm,5μm),流动相为乙腈-无水乙醇-3%磷酸溶液(80∶10∶10),流速1.0 mL/min,检测波长220 nm。结果苦参碱进样量在0.2064～3.048μg范围内与峰面积线性关系良好,回归方程Y=469.76X-3.2632(r=0.999 8),平均回收率为97.99%,RSD=1.21%(n=6)。结论所建立的高效液相色谱法简便、快速、重复性好,可用于苦参片的质量控制。
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| 关 键 词： | 高效液相色谱法 苦参片 苦参碱 含量测定 |
Content Determination of Matrine in Kushen Tablets |
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| Zhou Shiyu,Zhou Xiaoying,Tang Jingkun.Content Determination of Matrine in Kushen Tablets[J].China Pharmaceuticals,2011,20(7):27-28. |
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| Authors: | Zhou Shiyu Zhou Xiaoying Tang Jingkun |
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| Institution: | Zhou Shiyu1,Zhou Xiaoying1,Tang Jingkun2(1.Chengdu Institute for Drug Control,Sichuan,Chengdu,China 610045,2.Luzhou Medical College,Luzhou,China 646000) |
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| Abstract: | Objective To establish a method to determine the contents of matrine in Kushen Tablets.Methods An HPLC method was adopted. Alltech Alltima Amino column（250 mm×4.6 mm,5μm）was used.The mobile phase consisted of acetonitrile-anhydrous alcohol-3% phosphoric acid solution（80∶10∶10）at a flow rate of 1.0 mL/min;the wavelength of detector was 220 nm.Results The calibration curves of matrine showed good linearity in the range of 0.206 4-3.048μg.The regression equation was Y=469.76 X-3.263 2（r=0.999 8）.The average recovery rate was 97.99%,RSD=1.21%（n=6）.Conclusion This method is simple,high-speed and reproducible,and is suitable for the determination of matrine contents in Kushen Tablets. |
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| Keywords: | HPLC Kushen Tablets matrine content determination |
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