RP-HPLC法测定人血清中舒芬太尼的浓度

目的:建立测定人血清中舒芬太尼浓度的简便方法。方法:血清中舒芬太尼用乙酸乙酯-石油醚(4:1)单步提取后采用反相高效液相色谱法进行测定,其中色谱柱为Symmetry C18,流动相为乙腈-0.02mol.L-1醋酸铵-盐酸(pH4.3)(45:55),检测波长为230nm,柱温为室温,内标为艾司唑仑。结果:舒芬太尼血药浓度在0.025～4.000μg.mL-1范围内线性关系良好(r=0.9999);日内、日间RSD分别为3.49%～4.51%、4.67%～5.99%,平均回收率为101.92%～107.04%。结论:本方法提取步骤少,方法简单、快速、专一性强、准确,适合人血清中舒芬太尼的浓度监测。

Determination of Sufentanil Concentration in Human Serum by RP-HPLC

OBJECTIVE:To establish the method for the determination of sufentanil concentration in human serum.METHODS:Sufentanil was extracted from serum by ethyl ethanoate-petroleum ether(4:1) and determined by RP-HPLC.The determination was performed on Symmetry C18 column with mobile phase consisted of acetonitrile-0.02 mol·L-1 NH4Ac-HCL(pH4.3)(45:55).The detection wavelength was set at 230 nm and the column temperature was room temperature.Estazolam was used as internal standard.RESULTS:The linear range of sufentanil was 0.025～4.000 μg·mL-1(r=0.999 9) with intra-day and inter-day RSDs of 3.49%～4.51% and 4.67%～5.99%.Average recoveries were 101.92%～107.04%.CONCLUSION:The method is simple,rapid,accurate and specific with few steps.It is suitable for drug concentration monitoring of sufentanil in human serum.