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| 慢性强迫游泳应激抑郁模型大鼠海马钙离子浓度和c-fos表达的改变 | |
| 引用本文： | 刘昊,徐爱军,高俊玲,李海波,赵艳霞.慢性强迫游泳应激抑郁模型大鼠海马钙离子浓度和c-fos表达的改变[J].神经解剖学杂志,2010,26(1). |
| 作者姓名： | 刘昊 徐爱军 高俊玲 李海波 赵艳霞 |
| 作者单位： | 1. 华北煤炭医学院:附属医院神经内科,唐山,063000 2. 华北煤炭医学院:基础医学部,唐山,063000 3. 济南大学医学与生命科学学院,济南,250022 |
| 基金项目： | 河北省博士科研项目，卫生部人类疾病比较医学重点实验室开放课题基金 |
| 摘 要： | 目的:观察慢性强迫游泳应激模型大鼠海马区Ca2+]i和c-fos/Fos的变化,探讨慢性应激抑郁症与海马相关的发病机制。方法:选用雄性成年Wistar大鼠80只,将大鼠随机分为正常对照组(n=19)、急性对照组(n=12)和模型组(n=49),建立慢性强迫游泳应激抑郁模型,通过糖水偏好实验和开场实验对大鼠进行行为学测试。采用荧光分光光度法测定海马内Ca2+]i浓度,免疫印迹技术和逆转录-聚合酶链式反应检测Fos和c-fos的表达变化。结果:与正常对照组大鼠相比较:(1)模型组大鼠相对糖水消耗量、糖水偏好百分比、直立次数和体重增长率均降低(P0.01,P0.05);(2)模型组大鼠各个时间点海马神经元Ca2+]i增高(P0.01);(3)模型组大鼠海马Fos在应激后(0.5,1,2,4h)表达增强(P0.01),c-fosmRNA在应激后(0.5,1,2,4h)转录增强(P0.01)。与急性对照组相比较,模型组Fos蛋白表达高峰提前,表达量降低(P0.05)。结论:海马Ca2']i及c-fos/Fos的表达变化,可能参与了抑郁症的发病过程。 |
| 关 键 词： | 抑郁症 钙超载 海马 原癌基因 |
Changes of calicum and c-fos in hippocampus of chronic forced swimming stress model rats |
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| Liu Hao,Xu Aijun,Gao Junling,Li Haibo,Zhao Yanxia.Changes of calicum and c-fos in hippocampus of chronic forced swimming stress model rats[J].Chinese Journal of Neuroanatomy,2010,26(1). | |
| Authors: | Liu Hao Xu Aijun Gao Junling Li Haibo Zhao Yanxia |
| Institution: | Liu Hao1,Xu Aijun2,Gao Junling2,Li Haibo1,Zhao Yanxia3(1.Department of Neurology,Affiliated Hospital,2.Department of Basic Medical Sciences,North China Coal Medical College,Tangshan 063000,3.Institute of Medical Science , Life Science,Ji\'nan University,Ji\'nan 250022,China) |
| Abstract: | Objective:To observe the changes of intracellular free calcium concentration and the expression of c-fos in the hippocampus of chronic forced swimming stress rats and to study the pathogenesy of chronic depression.Methods:A total of 80 adult male Wistar rats were randomly divided into normal group,acute stress control group and model group.The behavior change was examined using sucrose preference test and open-filed test.The concentration of intracellular free calcium was detected by fluorescence spectropho... |
| Keywords: | depression calcium overload hippocampus immediate early gene |
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