天麻熄风口服液对大鼠右颈总动脉-左颈外静脉旁路血栓形成的影响

目的观察天麻熄风口服液对大鼠右颈总动脉-左颈外静脉旁路血栓形成的影响。方法取SD大鼠50只,随机分为5组,即空白对照组,天麻熄风低、中、高剂量组(生药含量分别为4、8、16 g/kg)及乙酰水杨酸组(生药含量为5 mg/kg),每组各10只。天麻熄风口服液低、中、高剂量组及乙酰水杨酸组分别按相应药量,容量按1 mL/100 g体质量灌胃给药,空白对照组予等容积蒸馏水灌胃,每日1次,连续7 d。末次给药前禁食12 h,给药后30 min,麻醉各组大鼠,分离右颈总动脉和左颈外静脉,从舌静脉注入肝素氯化钠溶液50 U/kg,观察各组大鼠右颈总动脉-左颈外静脉旁路血栓质量。另取50只大鼠,分组及给药方法、剂量同上,通过刺激电极损伤动脉内皮细胞,记录血栓形成时间。结果天麻熄风低、中、高剂量组及乙酰水杨酸组大鼠血栓质量均较空白对照组轻(P<0.01),血栓形成时间均较空白对照组大鼠延长(P<0.01),且天麻熄风高剂量组、乙酰水杨酸组大鼠血栓形成时间均较天麻熄风低、中剂量组延长(P<0.05)。结论天麻熄风口服液对右颈总动脉-左颈外静脉旁路血栓形成有显著的抑制作用。

Research of Tianmaxifeng oral liquid on thrombosis formation of the rat carotid artery - the left carotid vein bypass

Objective To explore the effect of Tianmaxifeng oral liquid on thrombosis formation of the rat carotid artery -the left carotid vein bypass. Methods 50 SD rats were randomly divided into five groups, control group ,Tianmaxifeng low- , middle- and high- dose group （crude drug 4,8,16 g/kg） and sulfacetic acid group （5 mg/kg）. Tianmaxifeng low -, middle - and high - dose group and sulfacetic acid group received relevant medicines intragastrically （capacity by 1 mL/100 g body weight）. Control group received equal volume of normal saline for 7 days. Fasted for 12 h before the last administration. At 30 min after administration the rats in each group were anesthe- tized, the right common carotid artery and left common carotid vein were separated. The quality of thrombosis of the rat carotid artery - the left carotid vein bypass was observed by the tongue vein injection of 50 U/kg heparin sodium chlo- ride solution. Arterial endothelial cells were treated by stimulating electrode damage, thrombus formation time was re- corded. Results The quality of rat thrombosis in Tianmaxifeng low -, middle - and high - dose group and sulfacetic acid group was lighter than that in control group （ P 〈 0.01 ）. Thrombosis time in these groups was delayed as com- pared with that in control group （P 〈 0.01 ）. Thrombosis time in Tianmaxifeng high - dose group and sulfacetic acid group was longer than that in Tianmaxifeng low - and middle - dose group （ P 〈 0.05 ）. Conclusion Tianmaxifeng oral liquid has significant inhibitory effect on thrombosis formation of carotid artery - the left carotid vein bypass