麦门冬汤对大鼠放射性肺损伤的预防作用研究

目的探讨麦门冬汤对放射性肺损伤的预防作用。方法将72只健康成年清洁级雌性Wistar大鼠随机分为空白对照组、单纯照射组、照射加中药组。开始照射前1周,空白对照、单纯照射组以蒸馏水2 mL/200 g灌胃,照射加中药组以每日2 mL/200 g中药灌胃,连续给予1周后,单纯照射、照射加中药组开始照射,连续照射5周。照射期间,每周测大鼠体质量1次,依照上述剂量,空白对照、单纯照射组以蒸馏水、照射加中药组以中药继续灌胃至照射结束。分别于照射开始后第5、12、26周末,各组随机抽取8只大鼠处死、取材,并检测比较大鼠一般情况、肺组织大体情况、右肺系数及肺组织HE染色检测情况。结果①放射性肺损伤大鼠自第2周开始出现精神萎靡、食欲下降、对刺激的反应性下降等症状,且随照射次数的增加,症状不断加重,而麦门冬汤对此变化具有拮抗作用;②放射性肺损伤大鼠在第5周末,肺系数明显高于正常大鼠,麦门冬汤可阻止放射性大鼠肺系数的增长;③肺组织大体解剖结构和HE染色后镜下观察发现,放射性照射导致大鼠肺组织在第5周末出现炎症反应,在12、26周末出现肺组织纤维化,麦门冬汤对此具有改善作用。结论采用麦门冬汤对大鼠放射性肺损伤进行先期干预治疗,可显著预防和改善大鼠的一般临床症状,减轻肺水肿程度,抑制或延缓肺组织出现炎症、纤维化,促进肺组织恢复,具有较好的预防和治疗放射性肺损伤的作用。

Prevention of Ophiopogon decoction on radiation pulmonary injury

Objective To research the prevention of Ophiopogon decoction on radiation pulmonary injury. Methods 72 healthy adult female Wistar rats were randomly divided into control group, the irradiation group, irra- diation and traditional Chinese medicine group. One week before irradiation, control group and the irradiation group received garage with 2 ml/200g of distilled water, traditional Chinese medicine group received 2 mL/200 g Chinese medicine daily gavage. After one week of continuous administration, the irradiation group and traditional Chinese medicine group received continuous exposure of five weeks. During exposure, body mass of rats were weekly meas- ured. In accordance with the above dose, control group and the irradiation group received distilled water, traditional Chinese medicine group received Chinese medicine garage to continue to the end of irradiation. 5, 12, 26 weekendsafter irradiation, general situation, lung tissue general, lung coefficient and lung tissue HE staining of rats were de- tected. Results Radiation - induced lung injury rats from the first two weeks appeared symptoms of listlessness, loss of appetite, decreased stimuli, with irradiation increased the number of symptoms has been increasing. Ophio- pogon decoction has the antagonism for this change. The lung coefficient in radiation - induced lung injury rats in the first five weeks was significantly higher than that of rats in control group, and Ophiopogon decoction can prevent the growth of the radioactive rat lung coefficient. Gross anatomical structure and HE staining show that inflammatory re-sponse appear in the first five weeks in rats after radiation exposure, pulmonary fibrosis in 12 and 26 weeks, Ophiopogon decoction can improve these status. Conclusion Early prophylactic treatment of radiation pulmonary injury in rats using Ophiopogon decoction can significantly prevent and improve the rat~s general clinical symptoms, reduce the degree of pulmonary edema, inhibit or slow the progressionof lung tissue inflammation, fibrosis, and promote lung tissue to restore function, has better prevention and treatment of radiation pulmonary injury.