当归中阿魏酸含量测定及质量标准的研究

目的参照高效液相色谱法(《中华人民共和国药典2010年版》一部附录ⅥD)试验,对当归中的主要成分阿魏酸进行含量测定。方法根据不同的流动相、提取方法、提取时间、取样量、提取溶剂、浓缩温度,最终确定最佳的测定方法,即以乙腈—0.1%磷酸(22∶78)为流动相;检测波长为324 nm,取样量为4 g,70%甲醇回流提取30 min,80℃的水浴加热的浓缩温度。结果根据实验并通过线性关系考察,证明线性关系良好。重复性、重现性、稳定性、回收率均符合要求。结论此方法当归以阿魏酸(C10H10O4)计,不少于0.10 mg,可作为以当归为主要成分的中药制剂的质量检测标准和参考依据。

Determination of ferulic acid in Angelica and research of quality standards

Objective To refer to high - performance liquid chromatography （ （Chinese Pharmacopoeia） 2010 of one appendix VI D） trial and determine the ferulic acid in the main components of Chinese angelica. Methods With different the mobile phase, extraction method, extraction time, sample volume, extraction solvent, concentration temperature, ultimately the best method for the determination was determined that Acetonitrile -0. 1% phosphoric acid （ 22 ： 78 ） as mobile phase ; detection wavelength is 324 nm, sample volume is 4 g,70% methanol extraction and 30 min, the temperature of concentrating is 80 ~C water bath heating. Results Through investigation proves the method of the linear relations is best. Repeatability ,reproducibility ,stability ,recovery rates are to meet requirements. Conclusion Angelica calculated in accordance with ferulic acid （C10H10O4 ） is not less than 0.10 mg/8 g and this method can be as quality inspection standards and reference of a main component for angelica of traditional Chinese medicine preparation.