成纤维细胞与肺癌细胞相互作用对膜型基质金属蛋白酶-1及基质金属蛋白酶-2表达的影响

目的 研究胚肺成纤维细胞对肺癌H460细胞膜型基质金属蛋白酶-1(MTl-MMP)、基质金属蛋白酶-2(MMP-2)表达的影响.方法 采用Western blot方法检测各组MT1-MMP的表达.取其上清,采用酶联免疫吸附法检测各组细胞培养液中活性MMP-2的浓度.结果 H460细胞、胚肺成纤维细胞单独培养时均有MT1-MMP表达,但混合培养后MT1-MMP表达增强(P＜0.05).H460细胞、胚肺成纤维细胞单独培养时MMP-2均有分泌,混合培养MMP-2分泌增强(P＜0.05).结论 胚肺成纤维细胞和肺癌H460细胞相互作用能通过上调MT1-MMP、MMP-2的表达从而促进肺癌的侵袭和转移,这可能为肺癌侵袭转移的一个重要机制.

Influence of interactions between fibroblust and lung cancer cell on membrane type 1-matrix metalloproteinase and matrix metalloproteuse-2 expression

Objective To investigate the influence of interactions between lung cancer cell H460and human fetal lung fibroblast on membrane type 1-matrix metalloproteinase (MT1-MMP) and matrix metalloprotease-2 (MMP-2) expression.Methods Western blot assay was used to analyse the expression of MT1-MMP protein in every group.Enzyme-linked immunosorbent assay was used to show the activation of MMP-2 in every group.Results Compared with those of the H460 group and HFL-1 group,MT1-MMP protein and MMP-2 protein of co-cultured group were enhanced ( P ＜0.05).Conclusions Fibroblast might increase the expression of MT1-MMP and MMP-2 activation in lung carcinoma cells,which can lead to the increased extracellularmatrix followed by acceleration of tumor cell migration and invasion.It is probably an key mechanism of lung carcinoma cell migration.