鲍曼不动杆菌外膜蛋白研究进展

鲍曼不动杆菌是医院获得性感染主要致病菌之一,其外膜蛋白是指肽聚糖外脂质双层中间镶嵌的一些特殊的蛋白质.CarO、33-to-36-kDa、OprD等外膜蛋白丢失与细菌耐药有关,而OmpA外膜蛋白是重要的致病因素.其研究技术分为提取、分离和鉴定3个方面,超声物理法、双向凝胶电泳、质谱技术及免疫印迹是目前常用技术.

Study on outer membrane proteins of Acinetobacter baumannii

Acinetobacter baumannii is one of the major pathogens in nosocomial infection,its outer membrane proteins are some special proteins embedded in lipid bilayer outside the peptidoglycan.The loss of CarO,33-to36-kDa,OprD and other outer membrane proteins is related with drug resistance,and outer membrane protein A is an important pathogenic factor.Its research technology including three aspects of the extraction,separation and identification,the ultrasound physical method,2-Dimensional Gel Electrophoresis,mass spectrometry and western blot analysis are commonly used now.