巢式PCR扩增SSU rRNA基因确诊1例三日疟原虫感染

目的 通过巢式PCR扩增18SSU rRNA基因片段确诊三日疟原虫的感染,减少三日疟的漏诊和误诊.方法 采用18SSU rRNA基因片段,通过巢式PCR方法,用镜检确诊的间日疟和恶性疟滤纸血样本进行对照,检测1例可疑疟疾患者滤纸血样本.结果 通过种间特异性引物的巢式PCR扩增后,间日疟及恶性疟确诊样本均扩增出相应的12...

Identification of a Plasmodium malariae infection case by nested PCR amplificaiton of SSUrRNA gene

Objective In order to reduce misseddiagnosis and misdiagnosis,Plasmodium malariae cases can be identified by nested-PCR amplification of 18SSU rRNA gene fragements.Methods Compared with the blood samples of P.vivax and P.falciparum,a suspicious febrile patient was tested by nested PCR amplification of 18SSU rRNA gene fragments.Results By using the interspecific primers amplification,specific fregaments were amplified from the blood samples of P.vivax and P.falciparum with 120 bp and 205bp respectively,and 144 bp from the suspicious febrile patient with Plasmodium malariae specific primers.All samples did not have the specific fregments of P.ovale.Conclusion Through nested-PCR amplification of 18SSU rRNA gene fragements,it was comfirmed that the patient was infected by P.malariae.