UPLC-QTOF-MS法同时测定春季文冠果叶中11种黄酮类化合物的含量

目的建立UPLC-QTOF-MS法同时测定春季文冠果叶中11种黄酮类化合物的含量。方法采用ACQUITY UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm),以体积分数0.05%甲酸水为流动相A,体积分数0.05%甲酸乙腈为流动相B,梯度洗脱,流速0.4 mL·min-1,电喷雾离子源(ESI),负离子全扫描模式,选择性离子监测定量方式进行。结果表没食子儿茶素,表儿茶素,杨梅素-3-O-芸香糖苷,芦丁,杨梅苷,山柰酚-3-O-芸香糖苷,槲皮苷,杨梅树皮素,山柰酚-3-O-α-吡喃鼠李糖苷,木犀草素和槲皮素质量浓度分别在0.38~38.00、0.46~46.00、0.90~90.00、0.75~75.00、1.60~160.00、0.45~45.00、0.60~60.00、0.016~1.60、0.30~30.00、0.010~1.00、0.010~1.00 g·L-1内与峰面积呈良好的线性关系,相关系数r均大于0.999。精密度、稳定性和重复性的RSD值均小于15%,平均回收率在91.5%~110.4%内,RSD小于6.5%。结论所建立的UPLC-QTOF-MS法适用于文冠果叶中黄酮类化合物的含量测定,为文冠果叶的合理应用提供依据。

Simultaneous determination of 11 flavonoids in spring leaves of Xanthoceras sorbifolia Bunge by UPLC-QTOF-MS

Objective To establish an UPLC-QTOF-MS method for simultaneous determination of 11 flavonoids in the leaves of Xanthoceras sorbifolia Bunge.Methods The mobile phase was performed on an ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8 μm) column,0.05 % formic acid as mobile phase A,acetonitrile(0.05 % formic acid,V/V) as mobile phase B,flow rate:0.4 mL·min-1,Detection was carried out by selective ion scanning equipped with an electrospray ionization source.Results The calibration curve was linear within the range of 0.38-38.00,0.46-46.00,0.90-90.00,0.75-75.00,1.60-160.00,0.45-45.00,0.60-60.00,0.016-1.60,0.30-30.00,0.010-1.00,0.010-1.00 mg·min-1 for epigallocatechin,epicatechin,myricitrin-3-O-rutinoside,rutin,myricetrin,kaempherol-3-O-rutinoside,quercitrin,myricetin,kaempherol-3-O-rhamnoside,luteolin and quercetin,respectively,and the correlation coefficient r was greater than 0.999.The RSD values of intra-day and inter-day precision,stability and repeatability were less than 15%,the average recovery was between 91.5% and 110.4%,and the RSD was less than 6.5%.Conclusion The established ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry is suitable for the determination of flavonoids in leaves of Xanthoceras sorbifolia Bunge,and provides a basis for the rational application of the leaves of Xanthoceras sorbifolia Bunge.