非小细胞肺癌患者癌组织中长链非编码RNA LINC01614表达及临床意义

目的:探讨非小细胞肺癌患者癌组织中长链非编码RNA LINC01614表达及临床意义。方法:选取2016年1月至2017年12月在鞍钢集团总医院行手术切除的75例原发性NSCLC患者癌组织为研究对象，并选取相对应癌旁组织(距肿瘤边缘>5 cm)作为对照，回顾性分析NSCLC相关lncRNA的表达，用实时荧光RT-PCR...

Expression and clinical significance of long non coding RNA LINC01614 in non-small cell lung cancer

Objective To investigate the expression and clinical significance of long non coding RNA LINC01614 in non-small cell lung cancer(NSCLC).Methods From January 2016 to December 2017,75 patients with primary NSCLC who underwent surgical resection in our hospital were selected as the cases,and the corresponding adjacent tissues(more than 5 from the tumor edge)were selected as control.The correlation between LINC01614 expression and clinicopathological characteristics of NSCLC patients was analyzed.ROC curve was drawn to determine the efficacy parameters of LINC01614 in the diagnosis of NSCLC.Kaplan Meier curve was used to analyze the survival analysis.Results The expression levels of 3261 lncRNAs were significantly different in NSCLC patients,including 1205 up-regulated and 2056 down-regulated.At the same time,LINC01614 was the most disordered lncRNA,and its expression was up-regulated in cancer tissues,22.92 times higher than that in paracancerous tissues.The higher the TNM stage,the lower the degree of tissue differentiation and the occurrence of lymph node metastasis,the higher the relative expression of LINC01614.The area under the ROC curve was 0.788(95%CI:0.710-0.821),with a diagnostic sensitivity of 58.62% and a specificity of 87.24%.With the relative expression of LINC01614 of 26.14 as the intercept,the overall survival of NSCLC patients in the high expression group(26 cases)and the low expression group(49 cases)was 22.2 months(95%CI:20.1-34.6)and 38.5 months(95%CI:25.3-45.5),respectively.The survival time of the high expression group was significantly lower than that of the low expression group(Z=10.248,P<0.05).Multivariate COX proportional hazards stepwise regression analysis showed that LINC01614 overexpression was an independent prognostic factor for NSCLC patients(HR=2.25,95%CI:1.30-3.90).Conclusions LINC01614 is highly expressed in NSCLC tissues,and is related to the malignant degree of NSCLC,which may have a certain guiding significance for the prognosis of NSCLC.